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AB237684

Anti-RNF128/GRAIL antibody

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(1 Publication)

Rabbit Polyclonal RNF128/GRAIL antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF128 aa 200-400.

View Alternative Names

E3 ubiquitin-protein ligase RNF128, Gene related to anergy in lymphocytes protein, RING finger protein 128, RING-type E3 ubiquitin transferase RNF128, GRAIL, RNF128

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RNF128/GRAIL antibody (AB237684)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNF128/GRAIL antibody (AB237684)

HepG2 (human epithelial cell line from cervix adenocarcinoma) cells stained for RNF128/GRAIL (green) using ab237684 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-RNF128/GRAIL antibody (AB237684)
  • WB

Supplier Data

Western blot - Anti-RNF128/GRAIL antibody (AB237684)

All lanes:

Western blot - Anti-RNF128/GRAIL antibody (ab237684) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF128 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q8TEB7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF128 also known as Gene Related to Anergy in Lymphocytes (GRAIL) is an E3 ubiquitin ligase with a molecular mass of approximately 44 kDa. This protein plays a mechanical role in tagging proteins with ubiquitin marking them for degradation by the proteasome. RNF128 is expressed in various tissues but it shows significant expression in immune cells particularly T cells. The protein contributes to regulating protein stability which is essential for maintaining cellular homeostasis and proper immune function.
Biological function summary

RNF128 is integral in regulating T cell anergy a state where T cells become unresponsive to antigenic stimulation. This protein helps maintain immune tolerance by suppressing excessive T cell responses preventing autoimmune reactions. RNF128 performs its biological function by being part of a complex that modulates signaling pathways involved in lymphocyte activation. Its expression and activity are modulated during immune responses regulating immune cell function and maintaining balance within the immune system.

Pathways

RNF128 is significantly involved in the ubiquitin-proteasome pathway impacting signal transduction and transcription processes. This protein is relevant in pathways related to immune tolerance particularly in controlling T cell receptor signaling. RNF128 interacts with other proteins like CD28 and CTLA-4 which are critical to T cell co-stimulatory and inhibitory pathways respectively. This interaction ensures the fine-tuning of immune responses preventing inappropriate activation that can lead to pathological states.

RNF128 has associations with autoimmune diseases and cancer. Its role in regulating immune tolerance links it to conditions like systemic lupus erythematosus where dysregulation can lead to an exaggerated immune response. Additionally RNF128's involvement in modulating T cell inactivity makes it relevant in certain cancers where immune evasion plays a critical role in disease progression. Through these conditions RNF128 remains connected with proteins like PD-1 which are involved in inhibitory pathways important for maintaining immune homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
See full target information E3 ubiquitin-protein ligase RNF128
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:4968-4980 PubMed36056909

2022

Risk stratification and prognosis prediction based on inflammation-related gene signature in lung squamous carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Zhai,Si Chen,Fangfang Duan,Junye Wang,Zerui Zhao,Yaobin Lin,Bingyu Rao,Yizhi Wang,Lie Zheng,Hao Long
View all publications
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