AB72533

Anti-RNF128/GRAIL antibody

Name: Anti-RNF128/GRAIL antibody (ab72533) | Abcam
Brand: Abcam Limited
SKU: AB72533
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal RNF128/GRAIL antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human E3 ubiquitin-protein ligase RNF128 aa 200-300.

View Alternative Names

E3 ubiquitin-protein ligase RNF128, Gene related to anergy in lymphocytes protein, RING finger protein 128, RING-type E3 ubiquitin transferase RNF128, GRAIL, RNF128

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF128/GRAIL antibody (AB72533)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF128/GRAIL antibody (AB72533)

Immunohistochemical staining of paraffin-embedded normal human brain tissue using ab72533 at 5 ug/ml (Panel A) and control rabbit Ig at 5 ug/ml (Panel B).

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Western blot - Anti-RNF128/GRAIL antibody (AB72533)

Endogenous RNF128 / GRAIL is reported to be detected at approximately 66 kD

All lanes:

Western blot - Anti-RNF128/GRAIL antibody (ab72533) at 1 µg/mL

Lane 1:

human spleen lysate

Lane 2:

human spleen lysate with immunising peptide

Lane 3:

mouse spleen lysate

Lane 4:

rat spleen lysate

Predicted band size: 47 kDa

Observed band size: 25 kDa,75 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human E3 ubiquitin-protein ligase RNF128 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q8TEB7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF128 also known as Gene Related to Anergy in Lymphocytes (GRAIL) is an E3 ubiquitin ligase with a molecular mass of approximately 44 kDa. This protein plays a mechanical role in tagging proteins with ubiquitin marking them for degradation by the proteasome. RNF128 is expressed in various tissues but it shows significant expression in immune cells particularly T cells. The protein contributes to regulating protein stability which is essential for maintaining cellular homeostasis and proper immune function.

Biological function summary

RNF128 is integral in regulating T cell anergy a state where T cells become unresponsive to antigenic stimulation. This protein helps maintain immune tolerance by suppressing excessive T cell responses preventing autoimmune reactions. RNF128 performs its biological function by being part of a complex that modulates signaling pathways involved in lymphocyte activation. Its expression and activity are modulated during immune responses regulating immune cell function and maintaining balance within the immune system.

Pathways

RNF128 is significantly involved in the ubiquitin-proteasome pathway impacting signal transduction and transcription processes. This protein is relevant in pathways related to immune tolerance particularly in controlling T cell receptor signaling. RNF128 interacts with other proteins like CD28 and CTLA-4 which are critical to T cell co-stimulatory and inhibitory pathways respectively. This interaction ensures the fine-tuning of immune responses preventing inappropriate activation that can lead to pathological states.

RNF128 has associations with autoimmune diseases and cancer. Its role in regulating immune tolerance links it to conditions like systemic lupus erythematosus where dysregulation can lead to an exaggerated immune response. Additionally RNF128's involvement in modulating T cell inactivity makes it relevant in certain cancers where immune evasion plays a critical role in disease progression. Through these conditions RNF128 remains connected with proteins like PD-1 which are involved in inhibitory pathways important for maintaining immune homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.

See full target information E3 ubiquitin-protein ligase RNF128

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:187 PubMed40253377

2025

RNF128 promotes gastric cancer progression by inhibiting autophagy-dependent ferroptosis through Beclin1 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenguo Zhu,Qishuai Chen,Siyi Song,Shi Peng,Huanxin Ding,Bingjun Li,Chuxuan Liu,Xin Jin,Linchuan Li,Jiankang Zhu,Guangyong Zhang

Immunity, inflammation and disease 11:e864 PubMed37249301

2023

RNF182 induces p65 ubiquitination to affect PDL1 transcription and suppress immune evasion in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xingdu Zeng,Xiaoyuan Tang,Xingxiang Chen,Huilan Wen

OncoTargets and therapy 13:10129-10141 PubMed33116595

2020

RNF128 Promotes Malignant Behaviors via EGFR/MEK/ERK Pathway in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Song Bai,Chi Zhang,Rui Peng,Guo-Qing Jiang,Sheng-Jie Jin,Qian Wang,Ai-Wu Ke,Dou-Sheng Bai

Translational oncology 11:116-124 PubMed29223109

2017

Genome-Wide Analysis of Barrett's Adenocarcinoma. A First Step Towards Identifying Patients at Risk and Developing Therapeutic Paths.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyang Dai,Qing Wang,Adrian Gonzalez Lopez,Mario Anders,Peter Malfertheiner,Michael Vieth,Wolfgang Kemmner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RNF128/GRAIL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF128/GRAIL antibody (AB72533)

Western blot - Anti-RNF128/GRAIL antibody (AB72533)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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