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Rabbit Polyclonal RNF13 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RNF13 aa 300 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF13 antibody - C-terminal (AB151601), expandable thumbnail
  • Western blot - Anti-RNF13 antibody - C-terminal (AB151601), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RNF13 antibody - C-terminal (AB151601), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human RNF13 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43567

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Target data

Function

E3 ubiquitin-protein ligase that regulates cell proliferation (PubMed:18794910, PubMed:23378536, PubMed:30595371). Involved in apoptosis regulation (PubMed:23378536, PubMed:30595371). Mediates ER stress-induced activation of JNK signaling pathway and apoptosis by promoting ERN1 activation and splicing of XBP1 mRNA (PubMed:23378536, PubMed:30595371). Also involved in protein trafficking and localization (PubMed:24387786).

Alternative names

Recommended products

Rabbit Polyclonal RNF13 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RNF13 aa 300 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human RNF13 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43567
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RNF13 also known as RING finger protein 13 is a protein encoded by the RNF13 gene. It has a molecular mass of approximately 30 kDa. RNF13 is an E3 ubiquitin ligase a type of enzyme that mediates the transfer of ubiquitin from an E2 enzyme to a substrate protein tagging it for degradation by the proteasome. This protein contains a RING finger domain which is essential for its ligase activity. RNF13 is mainly expressed in the brain liver and kidney suggesting its involvement in varied cellular functions across these tissues.

Biological function summary

RNF13 plays a role in modulating protein turnover by tagging specific proteins for degradation thereby maintaining cellular homeostasis. It is part of the ubiquitin-proteasome system (UPS) a complex that regulates protein degradation and recycling within cells. Through its E3 ligase activity RNF13 affects processes like cell signaling cell cycle and responses to stress. In addition to the UPS RNF13 interacts with other proteins including those involved in membrane trafficking influencing how cells communicate and transport molecules internally.

Pathways

RNF13 is significantly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway which removes misfolded or damaged proteins from the ER. It also participates in the Wnt signaling pathway a critical regulator of cell development and differentiation. In the context of the Wnt pathway RNF13 associates with proteins such as β-catenin influencing cell fate decisions. By connecting to these pathways RNF13 helps ensure proper cell growth and division acting as a molecular balance in cellular environments.

Associated diseases and disorders

RNF13's dysregulation links to neurodegenerative diseases and certain cancers. In neurodegenerative conditions abnormal RNF13 function can affect protein clearance leading to aggregation that harms neurons as seen in Alzheimer's disease. Furthermore its role in cancer emerges through its involvement in pathways associated with cell proliferation; disruptions in its normal activity can lead to malignant transformations. In these disorders RNF13 can interact with proteins like p53 which is important for tumor suppression highlighting its importance in disease management and therapy development.

Product promise

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3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF13 antibody - C-terminal (ab151601), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF13 antibody - C-terminal (ab151601)

    Immunohistochemical analysis of paraffin-embedded A549 xenograft, labeling RNF13 using ab151601 at 1/500 dilution.

  • Western blot - Anti-RNF13 antibody - C-terminal (ab151601), expandable thumbnail

    Western blot - Anti-RNF13 antibody - C-terminal (ab151601)

    10% SDS PAGE

    All lanes: Western blot - Anti-RNF13 antibody - C-terminal (ab151601) at 1/1000 dilution

    All lanes: 293T whole cell lysate at 30 µg

    Predicted band size: 43 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RNF13 antibody - C-terminal (ab151601), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RNF13 antibody - C-terminal (ab151601)

    Immunofluorescence analysis of paraformaldehyde-fixed MCF7, labeling RNF13 using ab151601 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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