Rabbit Polyclonal RNF135 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RNF135 aa 300 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RNF135 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IUD6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Target data
Function
E2-dependent E3 ubiquitin-protein ligase that functions as a RIGI coreceptor in the sensing of viral RNAs in cell cytoplasm and the activation of the antiviral innate immune response (PubMed:19017631, PubMed:19484123, PubMed:21147464, PubMed:23950712, PubMed:28469175, PubMed:31006531). Together with the UBE2D3, UBE2N and UB2V1 E2 ligases, catalyzes the 'Lys-63'-linked polyubiquitination of RIGI oligomerized on viral RNAs, an essential step in the activation of the RIG-I signaling pathway (PubMed:19017631, PubMed:21147464, PubMed:28469175, PubMed:31006531). Through a ubiquitin-independent parallel mechanism, which consists in bridging RIGI filaments forming on longer viral RNAs, further activates the RIG-I signaling pathway (PubMed:31006531). This second mechanism that synergizes with the ubiquitin-dependent one would thereby allow an RNA length-dependent regulation of the RIG-I signaling pathway (Probable). Associated with the E2 ligase UBE2N, also constitutively synthesizes unanchored 'Lys-63'-linked polyubiquitin chains that may also activate the RIG-I signaling pathway (PubMed:28469175, PubMed:31006531).
Alternative names
L13, RNF135, E3 ubiquitin-protein ligase RNF135, RIG-I E3 ubiquitin ligase, RING finger protein 135, RING finger protein leading to RIG-I activation, RING-type E3 ubiquitin transferase RNF135, REUL, Riplet
Recommended products
Rabbit Polyclonal RNF135 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RNF135 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RNF135 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IUD6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RNF135 also known as RING finger protein 135 is a protein with a mass of approximately 36.7 kDa. This protein is characterized by the presence of a RING-type zinc finger domain which facilitates its function as an E3 ubiquitin ligase a component important for tagging proteins with ubiquitin for degradation or regulation. RNF135 sees expression in various tissues with notably elevated levels in the brain and immune cells. Its gene location is on chromosome 17 providing additional insight into its genetic context.
- Biological function summary
The RING finger protein 135 participates in pathways related to protein homeostasis and cellular response. It functions in tagging certain substrates with ubiquitin part of a ubiquitination complex for proteasomal degradation. This action regulates processes such as apoptosis cell proliferation and DNA repair mechanisms. Such roles underline RNF135's significance in maintaining cellular function and genome integrity which are critical in complex multicellular organisms.
- Pathways
Research identifies RNF135 as an important player in the ubiquitin-proteasome pathway. This pathway is central to protein turnover and is regulated by other molecules like ubiquitin-conjugating enzymes and deubiquitinating enzymes. RNF135 also connects with the interferon signaling pathway linking it to immune response regulation. In both these pathways its interaction with proteins like ISG15 and TRIM25 impacts its function and regulatory effects further emphasizing its integrative role in cellular pathways.
- Associated diseases and disorders
Studies associate RNF135 with genetic disorders such as neurodevelopmental disorders and cancer. Alterations or dysregulation of its function can lead to conditions like chromosome 17q11.2 deletion syndrome impacting neural development and cognitive function. Besides RNF135 has been linked to various cancers likely due to its role in the ubiquitination of proteins controlling cell cycle and apoptosis. Proteins such as p53 a well-known tumor suppressor are connected to RNF135's regulatory network hinting at potential areas for therapeutic targeting in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-RNF135 antibody (ab229959)
All lanes: Western blot - Anti-RNF135 antibody (ab229959) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF135 antibody (ab229959)
Paraffin-embedded human gastric cancer tissue stained for RNF135 using ab229959 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF135 antibody (ab229959)
Paraffin-embedded human kidney tissue stained for RNF135 using ab229959 at 1/100 dilution in immunohistochemical analysis.
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