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AB89260

Anti-RNF144A antibody

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(5 Publications )

Mouse Polyclonal RNF144A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RNF144A.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RNF144A antibody (AB89260)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RNF144A antibody (AB89260)

ab89260 at 10μg/ml staining RNF144A on HeLa cell by immunofluorescence

Western blot - Anti-RNF144A antibody (AB89260)
  • WB

Unknown

Western blot - Anti-RNF144A antibody (AB89260)

All lanes:

Western blot - Anti-RNF144A antibody (ab89260) at 5 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 33 kDa

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Western blot - Anti-RNF144A antibody (AB89260)
  • WB

Unknown

Western blot - Anti-RNF144A antibody (AB89260)

All lanes:

Western blot - Anti-RNF144A antibody (ab89260) at 5 µg/mL

All lanes:

Rat brain tissue lysate at 50 µg

Predicted band size: 33 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RNF144A.

P50876

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF144A also known as IBRDC3 or MUL1 is an E3 ubiquitin-protein ligase with an approximate mass of 32 kDa. It plays an important role in tagging proteins with ubiquitin molecules marking them for degradation via the proteasome system. RNF144A comprises RING finger domains which facilitate its ligase activity. This protein expresses in a variety of tissues including the heart brain and liver among others. It interacts directly with substrates to ensure protein homeostasis which is important for cellular function.
Biological function summary

RNF144A influences cellular processes like apoptosis and cell proliferation. It is known to be a component of protein complexes involved in the regulation of these processes. By targeting specific proteins for degradation RNF144A helps control cellular growth and death signals which is essential in maintaining cellular equilibrium. Its activity can impact the levels of pro-apoptotic and anti-apoptotic factors which then influences the fate of the cell.

Pathways

RNF144A functions prominently in the ubiquitin-proteasome pathway and the JAK/STAT signaling pathway. Within the ubiquitin-proteasome pathway it determines the fate of misfolded or damaged proteins that need removal to prevent cellular dysfunction. In relation to the JAK/STAT signaling pathway it may influence cytokine signaling by regulating the turnover of pathway components. RNF144A's relationship with these pathways connects it to proteins like JAK kinases and STAT transcription factors which are important for immune responses and cellular signaling.

Dysfunction in RNF144A activity has links to cancer and neurodegenerative diseases. Aberrant activity of this protein can lead to uncontrolled cell growth which contributes to the development and progression of cancers such as breast cancer. Moreover in neurodegenerative diseases RNF144A may interact with proteins such as Parkin an E3 ligase affected in Parkinson’s disease affecting protein aggregation processes. Understanding RNF144A's roles in these conditions is important for developing targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed : 26216882). Mediates the ubiquitination and degradation of the DNA damage kinase PRKDC during DNA damage (PubMed : 24979766). Positively regulates DNA virus or exogenous cytosolic DNA-triggered innate immune response by mediating STING1 ubiquitination and increasing its 'Lys-6'-linked ubiquitination and translocation from the endoplasmic reticulum to the Golgi leading to downstream signaling pathways (PubMed : 37955227). Plays a positive role in EGF-dependent cell proliferation by prolonging EGF/EGFR signaling during EGF stimulation through EGFR ubiquitination (PubMed : 30171075). Increases ERK activity independently of EGFR signaling by promoting polyubiquitination and subsequent degradation of VRK3 in the cytosol (PubMed : 33067254).
See full target information RNF144A

Alternative Names

KIAA0161, RNF144, UBCE7IP4, RNF144A, E3 ubiquitin-protein ligase RNF144A, RING finger protein 144A, UbcM4-interacting protein 4, Ubiquitin-conjugating enzyme 7-interacting protein 4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 133: PubMed33067254

2020

RNF144a induces ERK-dependent cell death under oxidative stress via downregulation of vaccinia-related kinase 3.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Hyun Han,Kyong-Tai Kim

The Journal of biological chemistry 293:16307-16323 PubMed30171075

2018

RNF144A sustains EGFR signaling to promote EGF-dependent cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Shiuh-Rong Ho,Weei-Chin Lin

Nature medicine 23:1352-1361 PubMed29035367

2017

Targeting glioma stem cells through combined BMI1 and EZH2 inhibition.

Applications

WB

Species

Human

Xun Jin,Leo J Y Kim,Qiulian Wu,Lisa C Wallace,Briana C Prager,Tanwarat Sanvoranart,Ryan C Gimple,Xiuxing Wang,Stephen C Mack,Tyler E Miller,Ping Huang,Claudia L Valentim,Qi-Gang Zhou,Jill S Barnholtz-Sloan,Shideng Bao,Andrew E Sloan,Jeremy N Rich

The Journal of biological chemistry 290:23026-38 PubMed26216882

2015

Regulation of RNF144A E3 Ubiquitin Ligase Activity by Self-association through Its Transmembrane Domain.

Applications

Unspecified application

Species

Unspecified reactive species

Shiuh-Rong Ho,Yu-Ju Lee,Weei-Chin Lin

Proceedings of the National Academy of Sciences of 111:E2646-55 PubMed24979766

2014

RNF144A, an E3 ubiquitin ligase for DNA-PKcs, promotes apoptosis during DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Shiuh-Rong Ho,Christina S Mahanic,Yu-Ju Lee,Weei-Chin Lin
View all publications
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Product promise

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