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AB201212

Anti-RNF146 antibody

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(5 Publications)

Rabbit Polyclonal RNF146 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF146 aa 1-200.

View Alternative Names

E3 ubiquitin-protein ligase RNF146, Dactylidin, Iduna, RING finger protein 146, RING-type E3 ubiquitin transferase RNF146, RNF146

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF146 antibody (AB201212)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF146 antibody (AB201212)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid tissue labeling RNF146 with ab201212 at 1/100 dilution.

Western blot - Anti-RNF146 antibody (AB201212)
  • WB

Supplier Data

Western blot - Anti-RNF146 antibody (AB201212)

All lanes:

Western blot - Anti-RNF146 antibody (ab201212) at 1/1000 dilution

All lanes:

HeLa cell lysate

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF146 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NTX7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF146 also known as Iduna or dactylidin is an E3 ubiquitin ligase with a molecular mass of approximately 33 kDa. It plays an important role in the poly(ADP-ribose) (PAR)-dependent ubiquitination process adding ubiquitin to target proteins for degradation. RNF146 binds to PAR chains using its WWE domain triggering the ubiquitin-proteasome pathway. This protein is widely expressed in tissues notably in the brain heart and testis indicating its involvement in various cellular activities.
Biological function summary

RNF146 exhibits significant functions that influence cell survival and DNA repair. It interacts with PARP1 an important protein in the DNA damage response. As part of a larger protein complex RNF146 identifies and ubiquitinates proteins modified by PAR facilitating their recognition and subsequent degradation by the proteasome. Through these interactions RNF146 controls levels of damaged proteins and modulates the cellular response to stress contributing to genomic stability.

Pathways

RNF146 plays a part in the Wnt/β-catenin signaling pathway and the DNA damage response pathway. Within the Wnt signaling pathway RNF146 regulates Axin1 and other components influencing cell proliferation and differentiation. Additionally in the DNA damage pathway RNF146 aids in the fine-tuning of protein levels necessary for efficient repair by controlling PAR-modified proteins. Its interactions with proteins such as PARP1 and Tankyrase position RNF146 as a significant player in these pathways.

RNF146 has connections to neurodegenerative diseases and cancer. Abnormal RNF146 expression or activity can disrupt cellular homeostasis contributing to the pathogenesis of Alzheimer's disease through interactions with proteins like Tankyrase. In cancer altered RNF146 activity can influence tumorigenesis by modulating key signaling pathways such as the Wnt/β-catenin pathway. These links suggest that targeting RNF146 could offer therapeutic potential for both neurodegenerative diseases and cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation (PubMed : 21478859, PubMed : 21799911, PubMed : 22267412). May regulate many important biological processes, such as cell survival and DNA damage response (PubMed : 21825151, PubMed : 22267412). Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex (PubMed : 21478859, PubMed : 21799911). Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2 (PubMed : 21799911). Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation (PubMed : 21799911). Different ubiquitin linkage types have been observed : TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48' (PubMed : 21799911). May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location (PubMed : 21799911). Neuroprotective protein (PubMed : 21602803). Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos (By similarity). Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner (By similarity). Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest (By similarity). Promotes cell survival after gamma-irradiation (PubMed : 21825151). Facilitates DNA repair (PubMed : 21825151).
See full target information E3 ubiquitin-protein ligase RNF146
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:893888 PubMed35721496

2022

HIF-1/2α-Activated RNF146 Enhances the Proliferation and Glycolysis of Hepatocellular Carcinoma Cells via the PTEN/AKT/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guoliang Shen,Hao Wang,Ning Zhu,Qiliang Lu,Junwei Liu,Qiuran Xu,Dongsheng Huang

Folia neuropathologica 60:92-104 PubMed35359149

2022

Iduna contributes to the therapeutic effect of DHA in a cell and mouse model of traumatic brain injury via Wnt/MDM2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Shi,Fan Yang,Zhenyu Ge,Zongchun Tang,Kunhu Zhang,Bobo Chen,Hongbin Wang,Jian Hou,Hu Li,Zheng Tang

Nature communications 10:4363 PubMed31554794

2019

Tankyrase disrupts metabolic homeostasis and promotes tumorigenesis by inhibiting LKB1-AMPK signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Li,Yifan Wang,Shinya Neri,Yuanli Zhen,Lon Wolf R Fong,Yawei Qiao,Xu Li,Zhen Chen,Clifford Stephan,Weiye Deng,Rui Ye,Wen Jiang,Shuxing Zhang,Yonghao Yu,Mien-Chie Hung,Junjie Chen,Steven H Lin

Experimental cell research 383:111505 PubMed31326389

2019

NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jianning Xu,Zhiyong Sheng,Fuxin Li,Shu Wang,Ying Yuan,Meng Wang,Zhihong Yu

Cell research 28:918-933 PubMed30030518

2018

Circulating apoptotic bodies maintain mesenchymal stem cell homeostasis and ameliorate osteopenia via transferring multiple cellular factors.

Applications

Unspecified application

Species

Unspecified reactive species

Dawei Liu,Xiaoxing Kou,Chider Chen,Shiyu Liu,Yao Liu,Wenjing Yu,Tingting Yu,Ruili Yang,Runci Wang,Yanheng Zhou,Songtao Shi
View all publications
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