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Rabbit Recombinant Monoclonal RNF168 antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-RNF168 antibody [EPR24239-2] (AB271099), expandable thumbnail
  • Western blot - Anti-RNF168 antibody [EPR24239-2] (AB271099), expandable thumbnail
  • Western blot - Anti-RNF168 antibody [EPR24239-2] (AB271099), expandable thumbnail
  • Western blot - Anti-RNF168 antibody [EPR24239-2] (AB271099), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8-dependent H2A ubiquitination, promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Also involved in class switch recombination in immune system, via its role in regulation of DSBs repair. Following DNA damage, promotes the ubiquitination and degradation of JMJD2A/KDM4A in collaboration with RNF8, leading to unmask H4K20me2 mark and promote the recruitment of TP53BP1 at DNA damage sites. Not able to initiate 'Lys-63'-linked ubiquitination in vitro; possibly due to partial occlusion of the UBE2N/UBC13-binding region. Catalyzes monoubiquitination of 'Lys-13' and 'Lys-15' of nucleosomal histone H2A (H2AK13Ub and H2AK15Ub, respectively).

Alternative names

E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168

Recommended products

Rabbit Recombinant Monoclonal RNF168 antibody. Suitable for WB and reacts with Human samples.

Alternative names

E3 ubiquitin-protein ligase RNF168, hRNF168, RING finger protein 168, RING-type E3 ubiquitin transferase RNF168, RNF168

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24239-2

Purification technique

Affinity purification Protein A

Specificity

We recommend using freshly made lysate in western blot testing.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RNF168 also known as Ring Finger Protein 168 is an E3 ubiquitin ligase with a molecular mass of approximately 63 kDa. It functions primarily in the DNA damage response by adding ubiquitin to histones at sites of double-strand breaks. RNF168 is expressed in various tissues but is notably active in cells that experience DNA damage. This protein structure contains a RING finger domain which facilitates its role in ubiquitination.

Biological function summary

The actions of RNF168 are critical in maintaining genome stability and it serves as a part of the DNA damage signaling complex. It collaborates with other proteins in recognizing DNA damage and signaling repair processes. RNF168 amplifies the ubiquitin signal which then attracts repair proteins to the site of damage. This function places it as an important component in the cellular response to DNA breaks.

Pathways

RNF168 is an important player in the DNA damage response pathway and is particularly associated with the homologous recombination repair mechanism. It works alongside proteins such as 53BP1 and BRCA1 to initiate repair processes. RNF168’s ubiquitination activity facilitates the recruitment of these repair proteins coordinating the cellular response to DNA lesions and ensuring effective repair.

Associated diseases and disorders

RNF168 mutations or malfunctions have been linked to conditions like RIDDLE syndrome and certain cancers. In RIDDLE syndrome deficiencies in RNF168 disrupt the normal DNA repair processes leading to immunodeficiency radiosensitivity and learning difficulties. Cancer arises when the impaired DNA damage response fails to prevent mutations. Understanding RNF168 interactions particularly with proteins like 53BP1 and BRCA1 is essential for developing therapeutic strategies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099), expandable thumbnail

    Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: RNF168 knockdown HeLa whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

    Observed band size: 75 kDa

    This data was developed using ab271099, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes.

  • Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099), expandable thumbnail

    Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099) at 1/1000 dilution

    All lanes: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa

    Observed band size: 75 kDa

    This data was developed using ab271099, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099), expandable thumbnail

    Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    We recommend using freshly made lysate in western blot testing.

    All lanes: Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099) at 1/1000 dilution

    Lane 1: Frozen MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Fresh MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa

    Observed band size: 75 kDa

    Exposure time: 20s

  • Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099), expandable thumbnail

    Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    We recommend using freshly made lysate in western blot testing.

    All lanes: Western blot - Anti-RNF168 antibody [EPR24239-2] (ab271099) at 1/1000 dilution

    Lane 1: Frozen HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate at 20 µg

    Lane 2: Fresh HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 65 kDa

    Observed band size: 75 kDa

    Exposure time: 7s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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