AB197321

Anti-RNF183 antibody

Name: Anti-RNF183 antibody (ab197321) | Abcam
Brand: Abcam Limited
SKU: AB197321
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal RNF183 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RNF183 aa 1 to C-terminus.

View Alternative Names

E3 ubiquitin-protein ligase RNF183, RNF183

1 Images

Supplier Data

Western blot - Anti-RNF183 antibody (AB197321)

All lanes:

Western blot - Anti-RNF183 antibody (ab197321) at 1/1000 dilution

All lanes:

HepG2 cell lysate

Predicted band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human RNF183 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96D59

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF183 also known as RING finger protein 183 is an E3 ubiquitin ligase. It is approximately 28 kDa in mass and belongs to the RING finger protein family characterized by a specific motif for binding zinc ions. RNF183 directs the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to specific substrates marking these proteins for proteasomal degradation or altering their cellular functions. This protein is expressed mainly in the kidney and small intestine with some presence in other tissues.

Biological function summary

The RNF183 protein regulates protein degradation cellular signaling and stability. It often acts independently and not as part of a larger protein complex influencing various cellular processes. An important function includes regulating pathways associated with immune responses and inflammatory signaling essential for maintaining cell homeostasis and immune system balance. Through its activity RNF183 modulates specific proteins involved in cellular signaling cascades.

Pathways

RNF183 mainly participates in ubiquitin-proteasome and inflammatory response pathways. It interacts with other proteins such as TNF receptor-associated factors which are important for relaying information to downstream signaling molecules. In the context of the NF-kB signaling pathway RNF183 influences this important immune pathway by dictating the stability and activity of various signaling proteins affecting immune response and inflammation regulation.

Studies have linked altered RNF183 expression to digestive system disorders and certain cancers such as gastric cancer. Changes in RNF183 levels can disrupt normal cellular signaling contributing to tumorigenesis and disease progression. The interaction with TRAF proteins and other components within the affected pathways underlines its role in disease mechanisms providing potential therapeutic targets for research and drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Acts as an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed : 29507230). Triggers apoptosis in response to prolonged ER stress by mediating the polyubiquitination and subsequent proteasomal degradation of BCL2L1 (PubMed : 29507230). May collaborate with FATE1 to restrain BIK protein levels thus regulating apoptotic signaling (PubMed : 26567849).

See full target information RNF183

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 55:1145-1158 PubMed37258572

2023

The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Yan,Xurui Li,Mou Peng,Yali Zuo,Yinhuai Wang,Pian Liu,Weigang Ren,Xin Jin

Cell death & disease 8:e2994 PubMed28796265

2017

RNF183 promotes proliferation and metastasis of colorectal cancer cells via activation of NF-κB-IL-8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Geng,Xin Tan,Jiangxue Wu,Zhizhong Pan,Min Yi,Wei Shi,Ranyi Liu,Chen Yao,Gaoyuan Wang,Jiaxin Lin,Lin Qiu,Wenlin Huang,Shuai Chen

Chinese journal of cancer 36:63 PubMed28756770

2017

Synthetic lethal short hairpin RNA screening reveals that ring finger protein 183 confers resistance to trametinib in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Geng,Xin Tan,Zhixiang Zuo,Jiangxue Wu,Zhizhong Pan,Wei Shi,Ranyi Liu,Chen Yao,Gaoyuan Wang,Jiaxin Lin,Lin Qiu,Wenlin Huang,Shuai Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RNF183 antibody

Western blot - Anti-RNF183 antibody (AB197321)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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