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AB181999

Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal RNF185 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

E3 ubiquitin-protein ligase RNF185, RING finger protein 185, RNF185

3 Images
Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)
  • IP

Supplier Data

Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181999 at 1/40 dilution (lane 1) or a negative control (lane 2) followed by Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (ab181999)

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Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)
  • WB

Supplier Data

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)

All lanes:

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (ab181999) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

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Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)
  • WB

Lab

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (ab181999) at 1/10000 dilution

Lane 1:

Human RNF185 full-length ORF recombinant protein with GST-tag at N-terminal at 10 µg

Lane 2:

Human RNF5 full-length ORF recombinant protein with GST-tag at N-terminal at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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Exposure time: 1s

  • Carrier free

    Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14070-94

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF185 also known as RING finger protein 185 is an E3 ubiquitin-protein ligase that plays an important role in the ubiquitination process. It works with RNF5/NG2 another protein and both belong to the RING finger protein family which is characterized by a specific domain that facilitates protein-protein interactions. This protein has a mass of approximately 35 kDa. RNF185 is expressed across various tissues with higher levels often noted in the heart and skeletal muscle indicating a role in cell function within these tissues.
Biological function summary

RNF185 regulates protein homeostasis by marking target proteins for degradation via ubiquitination. It does not act alone; often it forms part of a larger complex that includes components like E2 ubiquitin-conjugating enzymes. This activity ensures that cells maintain the proper levels of proteins helping remove misfolded or damaged proteins. The complex activity of RNF185 is essential for cellular quality control mechanisms maintaining balance within the protein environment of the cell.

Pathways

Involvement of RNF185 connects it to important cellular processes including the endoplasmic reticulum-associated degradation (ERAD) pathway and the Wnt signaling pathway. In ERAD RNF185 collaborates with other proteins such as RNF5 to facilitate degradation of misfolded proteins in the endoplasmic reticulum. Through the Wnt pathway control of protein levels by RNF185 influences cellular proliferation and differentiation processes that are essential for proper tissue development and homeostasis.

RNF185 shows significant links to cardiovascular diseases and some forms of cancer. Dysregulation of RNF185 and its interaction with proteins like RNF5 can lead to abnormal protein accumulation or degradation contributing to pathogenesis in these conditions. In cardiovascular diseases improper protein turnover affecting heart and muscle tissues can lead to cardiac dysfunction. In cancer altered activity of RNF185 may affect cell growth pathways promoting tumor growth and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed : 21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed : 27485036). Protects cells from ER stress-induced apoptosis (PubMed : 27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed : 24019521). Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity (PubMed : 28273161). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed : 24019521).
See full target information RNF185

Additional targets

RNF5
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 14:335-347 PubMed38618242

2024

Hapln1 promotes dedifferentiation and proliferation of iPSC-derived cardiomyocytes by promoting versican-based GDF11 trapping.

Applications

Unspecified application

Species

Unspecified reactive species

Ding-Jun Hao,Yue Qin,Shi-Jie Zhou,Bu-Huai Dong,Jun-Song Yang,Peng Zou,Li-Ping Wang,Yuan-Ting Zhao

Nutrition & metabolism 21:5 PubMed38167476

2024

D-mannose promotes the degradation of IDH2 through upregulation of RNF185 and suppresses breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruonan Zhang,Ziyin Tian,Yanping Xu,Lei Lv

Autophagy 20:1286-1313 PubMed38084826

2023

Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185.

Applications

Unspecified application

Species

Unspecified reactive species

Meirong Chen,Xin Zhang,Fanshu Kong,Peng Gao,Xinna Ge,Lei Zhou,Jun Han,Xin Guo,Yongning Zhang,Hanchun Yang

Cell reports 41:111675 PubMed36417855

2022

Dynamic quality control machinery that operates across compartmental borders mediates the degradation of mammalian nuclear membrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Ling Tsai,Christopher J F Cameron,Maria Fernanda Forni,Renee R Wasko,Brigitte S Naughton,Valerie Horsley,Mark B Gerstein,Christian Schlieker

Molecular cell 82:4277-4289.e10 PubMed36283413

2022

ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Benjamin M Adams,Vincent Chu,Joao A Paulo,Sichen Shao

EMBO reports 23:e54859 PubMed35979733

2022

UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zheng,Yu Cao,Jun Yang,Hui Jiang

Molecular cell 79:768-781.e7 PubMed32738194

2020

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Michael L van de Weijer,Logesvaran Krshnan,Sabrina Liberatori,Elena Navarro Guerrero,Jacob Robson-Tull,Lilli Hahn,Robert Jan Lebbink,Emmanuel J H J Wiertz,Roman Fischer,Daniel Ebner,Pedro Carvalho
View all publications
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Product promise

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