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AB250564

Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free

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Rabbit Recombinant Monoclonal RNF185 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

E3 ubiquitin-protein ligase RNF185, RING finger protein 185, RNF185, G16, NG2, RMA1, RNF5, E3 ubiquitin-protein ligase RNF5, RING finger protein 5, Ram1 homolog, HsRma1

3 Images
Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free (AB250564)
  • IP

Supplier Data

Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free (AB250564)

This data was developed using ab181999, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181999 at 1/40 dilution (lane 1) or a negative control (lane 2) followed by Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (<a href='/en-us/products/primary-antibodies/rnf185-rnf5-ng2-antibody-epr14070-94-ab181999'>ab181999</a>)

false

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free (AB250564)
  • WB

Supplier Data

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free (AB250564)

This data was developed using ab181999, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (<a href='/en-us/products/primary-antibodies/rnf185-rnf5-ng2-antibody-epr14070-94-ab181999'>ab181999</a>) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

false

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free (AB250564)
  • WB

Lab

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free (AB250564)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (<a href='/en-us/products/primary-antibodies/rnf185-rnf5-ng2-antibody-epr14070-94-ab181999'>ab181999</a>) at 1/10000 dilution

Lane 1:

Human RNF185 full-length ORF recombinant protein with GST-tag at N-terminal at 10 µg

Lane 2:

Human RNF5 full-length ORF recombinant protein with GST-tag at N-terminal at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 1s

  • Unconjugated

    Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14070-94

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB181999

Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94]

RabMAb|Recombinant

Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)

  • 5
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Primary Antibodies

AB250443

Anti-RNF185+RNF5/NG2 antibody [EPR14070] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] - BSA and Azide free (AB250443)

  • 0
  • 0
Primary Antibodies

AB308066

Anti-RNF5/NG2 antibody [EPR27133-30]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-RNF5/NG2 antibody [EPR27133-30] (AB308066)

  • 0
  • 0
Primary Antibodies

AB249958

Anti-Histone H3 (formyl K122) antibody [EPR17896] - BSA and Azide free

RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (formyl K122) antibody [EPR17896] - BSA and Azide free (AB249958)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250564 is the carrier-free version of ab181999.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF185 also known as RING finger protein 185 is an E3 ubiquitin-protein ligase that plays an important role in the ubiquitination process. It works with RNF5/NG2 another protein and both belong to the RING finger protein family which is characterized by a specific domain that facilitates protein-protein interactions. This protein has a mass of approximately 35 kDa. RNF185 is expressed across various tissues with higher levels often noted in the heart and skeletal muscle indicating a role in cell function within these tissues.
Biological function summary

RNF185 regulates protein homeostasis by marking target proteins for degradation via ubiquitination. It does not act alone; often it forms part of a larger complex that includes components like E2 ubiquitin-conjugating enzymes. This activity ensures that cells maintain the proper levels of proteins helping remove misfolded or damaged proteins. The complex activity of RNF185 is essential for cellular quality control mechanisms maintaining balance within the protein environment of the cell.

Pathways

Involvement of RNF185 connects it to important cellular processes including the endoplasmic reticulum-associated degradation (ERAD) pathway and the Wnt signaling pathway. In ERAD RNF185 collaborates with other proteins such as RNF5 to facilitate degradation of misfolded proteins in the endoplasmic reticulum. Through the Wnt pathway control of protein levels by RNF185 influences cellular proliferation and differentiation processes that are essential for proper tissue development and homeostasis.

RNF185 shows significant links to cardiovascular diseases and some forms of cancer. Dysregulation of RNF185 and its interaction with proteins like RNF5 can lead to abnormal protein accumulation or degradation contributing to pathogenesis in these conditions. In cardiovascular diseases improper protein turnover affecting heart and muscle tissues can lead to cardiac dysfunction. In cancer altered activity of RNF185 may affect cell growth pathways promoting tumor growth and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed : 21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed : 27485036). Protects cells from ER stress-induced apoptosis (PubMed : 27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed : 24019521). Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity (PubMed : 28273161). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed : 24019521).
See full target information RNF185

Additional targets

RNF5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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