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AB250443

Anti-RNF185+RNF5/NG2 antibody [EPR14070] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RNF185 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

E3 ubiquitin-protein ligase RNF185, RING finger protein 185, RNF185, G16, NG2, RMA1, RNF5, E3 ubiquitin-protein ligase RNF5, RING finger protein 5, Ram1 homolog, HsRma1

2 Images
Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] - BSA and Azide free (AB250443)
  • WB

Supplier Data

Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] - BSA and Azide free (AB250443)

This data was developed using ab181227, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] (<a href='/en-us/products/primary-antibodies/rnf185rnf5-ng2-antibody-epr14070-ab181227'>ab181227</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] - BSA and Azide free (AB250443)
  • WB

Supplier Data

Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] - BSA and Azide free (AB250443)

Blocking buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-RNF185+RNF5/NG2 antibody [EPR14070] (<a href='/en-us/products/primary-antibodies/rnf185rnf5-ng2-antibody-epr14070-ab181227'>ab181227</a>) at 1/1000 dilution

Lane 1:

Human RNF185 full-length ORF recombinant protein with GST-tag at N-terminal at 0.01 µg

Lane 2:

Human RNF5 full-length ORF recombinant protein with GST-tag at N-terminal at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 47.45 kDa

true

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14070

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB181999

Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94]

RabMAb|Recombinant

Immunoprecipitation - Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] (AB181999)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Secondary Antibodies

AB99625

Rat monoclonal [JC5-1] Anti-Mouse lambda light chain (HRP)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250443 is the carrier-free version of ab181227.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RNF185 and RNF5/NG2 proteins function as E3 ubiquitin ligases which add ubiquitin to substrate proteins and mark them for degradation by the proteasome. Together these proteins play an important role in the endoplasmic reticulum-associated degradation (ERAD) pathway. RNF185 is also known as Ring Finger Protein 185 while RNF5 is often referred to as Ring Finger Protein 5 or NG2 in some contexts. RNF185 has a mass of approximately 34 kDa and RNF5 has a mass of about 22 kDa. These proteins express primarily in tissues such as the heart liver and kidneys participating in cellular quality control and stress responses.
Biological function summary

These ubiquitin ligases mediate protein homeostasis by regulating the turnover of misfolded or damaged proteins through the ubiquitin-proteasome system. They interact with several other proteins to form a complex involved in tagging defective molecules. This complex helps maintain cellular integrity by preventing the accumulation of potentially toxic proteins. RNF185 and RNF5 also regulate cellular responses under stress conditions revealing their intervention in adaptive mechanisms of cells to maintain survival when under duress.

Pathways

RNF185 and RNF5 coordinate within the ERAD pathway and contribute to protein quality control by linking recognition of misfolded proteins to proteasomal degradation. They relate closely with proteins such as Ubiquitin-Conjugating Enzyme E2 (UBE2) and Derlin-1 integral players in the ERAD process. Their participation in this pathway is fundamental for removing defective proteins therefore upholding cellular function in stress environments. Both RNF185 and RNF5 influence pathways like the Unfolded Protein Response (UPR) further highlighting their importance in maintaining protein balance and cellular health.

Defects or dysregulation in RNF185 and RNF5 expression have implications in conditions like neurodegenerative diseases and cancer. Aberrant activity of these proteins may lead to the accumulation of misfolded proteins or disruption of normal cellular processes contributing to disease pathology. For instance in the context of cancer these proteins interact with components such as the tumor suppressor protein p53 affecting cell cycle regulation and apoptosis. Their involvement in neurodegenerative disorders may be linked to erroneous removal of proteins contributing to neuronal damage and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1 (PubMed : 21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed : 27485036). Protects cells from ER stress-induced apoptosis (PubMed : 27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed : 24019521). Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity (PubMed : 28273161). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed : 24019521).
See full target information RNF185

Additional targets

RNF5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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