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AB181104

Anti-RNF20 antibody [EPR13562(B)]

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(4 Publications)

Rabbit Recombinant Monoclonal RNF20 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

BRE1A, RNF20, E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, RING finger protein 20, RING-type E3 ubiquitin transferase BRE1A

4 Images
Flow Cytometry (Intracellular) - Anti-RNF20 antibody [EPR13562(B)] (AB181104)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RNF20 antibody [EPR13562(B)] (AB181104)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling RNF20 with ab181104 at 1/290 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody [EPR13562(B)] (AB181104)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody [EPR13562(B)] (AB181104)

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling RNF20 with ab181104 at 1/200 (8.5 μg/ml) dilution, followed by ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

Immunoprecipitation - Anti-RNF20 antibody [EPR13562(B)] (AB181104)
  • IP

Supplier Data

Immunoprecipitation - Anti-RNF20 antibody [EPR13562(B)] (AB181104)

Western blot analysis on immunoprecipitation of 293 cell lysate labeling RNF20 using ab181104 at a 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution and 5% NFDM/TBST blocking peptide.

All lanes:

Immunoprecipitation - Anti-RNF20 antibody [EPR13562(B)] (ab181104)

Predicted band size: 114 kDa

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Western blot - Anti-RNF20 antibody [EPR13562(B)] (AB181104)
  • WB

Supplier Data

Western blot - Anti-RNF20 antibody [EPR13562(B)] (AB181104)

All lanes:

Western blot - Anti-RNF20 antibody [EPR13562(B)] (ab181104) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

MCF-7 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 114 kDa

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  • Carrier free

    Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13562(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB181032

Anti-RNF20 antibody [EPR13563(B)]

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/290", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF20 also known as BRE1A or E3 ubiquitin-protein ligase RNF20 mechanically functions as an E3 ligase that mediates ubiquitination. Its mass is approximately 110 kDa. RNF20 is expressed across various tissues with a high presence in the spleen and testis. It specifically adds ubiquitin to lysine residues on target histones leading to changes in chromatin structure and gene expression.
Biological function summary

RNF20 works within the ubiquitination system and forms part of the RNF20/RNF40 complex. This complex plays a role in the monoubiquitination of histone H2B at lysine 120 (H2BK120ub) a significant post-translational modification influencing transcriptional regulation. The modification aids in recruiting downstream effectors and is important for gene expression regulation and DNA damage repair mechanisms.

Pathways

RNF20 interacts with several cellular processes including the DNA damage response and transcriptional activation pathways. It significantly contributes to the DNA repair pathway by facilitating recruitment of repair proteins and is also involved in transcription regulation through the histone modification pathway. Furthermore RNF20 shows a functional relationship with RNF40 another E3 ligase in these pathways emphasizing its integrative role in cellular functioning.

Disruptions in RNF20 activity have connections to cancer and neurodegenerative disorders. Altered RNF20 expression or mutations can influence tumor growth due to impaired regulation of gene expression and DNA repair impacting proteins like p53. Additionally in neurodegenerative disorders anomalies in RNF20-mediated ubiquitination can affect protein homeostasis linking to proteins involved in neuronal integrity and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.. (Microbial infection) Promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing the expression of lytic viral genes including the latency switch gene RTA/ORF50.
See full target information RNF20
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 31:568-585 PubMed36910712

2023

BMAL1-TTK-H2Bub1 loop deficiency contributes to impaired BM-MSC-mediated bone formation in senile osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Jinteng,Xu Peitao,Yu Wenhui,Ye Guiwen,Ye Feng,Xu Xiaojun,Su Zepeng,Lin Jiajie,Che Yunshu,Zhang Zhaoqiang,Zeng Yipeng,Li Zhikun,Feng Pei,Cao Qian,Li Dateng,Xie Zhongyu,Wu Yanfeng,Shen Huiyong

Cancers 15: PubMed36612252

2022

Exploiting the Endogenous Ubiquitin Proteasome System in Targeted Cancer Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Noa Hauser,Joud Hirbawi,Meshi Saban Golub,Samar Zabit,Michal Lichtenstein,Haya Lorberboum-Galski

The Journal of biological chemistry 295:18485-18493 PubMed33097595

2020

Phosphorylation of SMURF2 by ATM exerts a negative feedback control of DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Ya Tang,Adam Thomas,Ming Zhou,Ying E Zhang

PloS one 12:e0168960 PubMed28045951

2017

An Experimental Analysis of the Molecular Effects of Trastuzumab (Herceptin) and Fulvestrant (Falsodex), as Single Agents or in Combination, on Human HR+/HER2+ Breast Cancer Cell Lines and Mouse Tumor Xenografts.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Chen,Ziyi Weng,Yunshu Lu,Yijun Jia,Longlong Ding,Fang Bai,Meixin Ge,Qing Lin,Kejin Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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