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Rabbit Recombinant Monoclonal RNF20 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (AB250351), expandable thumbnail
  • Immunoprecipitation - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (AB250351), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (AB250351), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (AB250351), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. (Microbial infection) Promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing the expression of lytic viral genes including the latency switch gene RTA/ORF50.

Alternative names

BRE1A, RNF20, E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, RING finger protein 20, RING-type E3 ubiquitin transferase BRE1A

Recommended products

Rabbit Recombinant Monoclonal RNF20 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR13562(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250351 is the carrier-free version of Anti-RNF20 antibody [EPR13562(B)] ab181104.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RNF20 also known as BRE1A or E3 ubiquitin-protein ligase RNF20 mechanically functions as an E3 ligase that mediates ubiquitination. Its mass is approximately 110 kDa. RNF20 is expressed across various tissues with a high presence in the spleen and testis. It specifically adds ubiquitin to lysine residues on target histones leading to changes in chromatin structure and gene expression.

Biological function summary

RNF20 works within the ubiquitination system and forms part of the RNF20/RNF40 complex. This complex plays a role in the monoubiquitination of histone H2B at lysine 120 (H2BK120ub) a significant post-translational modification influencing transcriptional regulation. The modification aids in recruiting downstream effectors and is important for gene expression regulation and DNA damage repair mechanisms.

Pathways

RNF20 interacts with several cellular processes including the DNA damage response and transcriptional activation pathways. It significantly contributes to the DNA repair pathway by facilitating recruitment of repair proteins and is also involved in transcription regulation through the histone modification pathway. Furthermore RNF20 shows a functional relationship with RNF40 another E3 ligase in these pathways emphasizing its integrative role in cellular functioning.

Associated diseases and disorders

Disruptions in RNF20 activity have connections to cancer and neurodegenerative disorders. Altered RNF20 expression or mutations can influence tumor growth due to impaired regulation of gene expression and DNA repair impacting proteins like p53. Additionally in neurodegenerative disorders anomalies in RNF20-mediated ubiquitination can affect protein homeostasis linking to proteins involved in neuronal integrity and survival.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351), expandable thumbnail

    Western blot - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351)

    This data was developed using Anti-RNF20 antibody [EPR13562(B)] ab181104, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RNF20 antibody [EPR13562(B)] (Anti-RNF20 antibody [EPR13562(B)] ab181104) at 1/5000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: 293 cell lysate at 20 µg

    Lane 3: MCF-7 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 114 kDa

  • Immunoprecipitation - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351), expandable thumbnail

    Immunoprecipitation - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351)

    This data was developed using Anti-RNF20 antibody [EPR13562(B)] ab181104, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation of 293 cell lysate labeling RNF20 using Anti-RNF20 antibody [EPR13562(B)] ab181104 at a 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution and 5% NFDM/TBST blocking peptide.

    All lanes: Immunoprecipitation - Anti-RNF20 antibody [EPR13562(B)] (Anti-RNF20 antibody [EPR13562(B)] ab181104)

    Predicted band size: 114 kDa

  • Flow Cytometry (Intracellular) - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351)

    This data was developed using Anti-RNF20 antibody [EPR13562(B)] ab181104, the same antibody clone in a different buffer formulation.

    Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat cells labeling RNF20 with Anti-RNF20 antibody [EPR13562(B)] ab181104 at 1/290 dilution (red) or a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at a 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody [EPR13562(B)] - BSA and Azide free (ab250351)

    RNF20 Immunocytochemistry/ Immunofluorescence staining of MCF7 (human breast adenocarcinoma epithelial cell) using rabbit Anti-RNF20 antibody

    Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling RNF20 with Anti-RNF20 antibody [EPR13562(B)] ab181104 at 1/200 (8.5 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

    This data was developed using Anti-RNF20 antibody [EPR13562(B)] ab181104, the same antibody clone in a different buffer formulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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