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AB250297

Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RNF20 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BRE1A, RNF20, E3 ubiquitin-protein ligase BRE1A, BRE1-A, hBRE1, RING finger protein 20, RING-type E3 ubiquitin transferase BRE1A

3 Images
Western blot - Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free (AB250297)
  • WB

Supplier Data

Western blot - Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free (AB250297)

This data was developed using ab181032, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF20 antibody [EPR13563(B)] (<a href='/en-us/products/primary-antibodies/rnf20-antibody-epr13563b-ab181032'>ab181032</a>) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 114 kDa

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Flow Cytometry (Intracellular) - Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free (AB250297)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free (AB250297)

This data was developed using ab181032, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling RNF20 with ab181032 (red) at 1/10 dilution or a rabbit IgG (negative) (green).

Immunoprecipitation - Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free (AB250297)
  • IP

Supplier Data

Immunoprecipitation - Anti-RNF20 antibody [EPR13563(B)] - BSA and Azide free (AB250297)

This data was developed using ab181032, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from 293 cell lysate labeling RNF20 with ab181032 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RNF20 antibody [EPR13563(B)] (<a href='/en-us/products/primary-antibodies/rnf20-antibody-epr13563b-ab181032'>ab181032</a>)

Predicted band size: 114 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13563(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250297 is the carrier-free version of ab181032.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF20 also known as BRE1A or E3 ubiquitin-protein ligase RNF20 mechanically functions as an E3 ligase that mediates ubiquitination. Its mass is approximately 110 kDa. RNF20 is expressed across various tissues with a high presence in the spleen and testis. It specifically adds ubiquitin to lysine residues on target histones leading to changes in chromatin structure and gene expression.
Biological function summary

RNF20 works within the ubiquitination system and forms part of the RNF20/RNF40 complex. This complex plays a role in the monoubiquitination of histone H2B at lysine 120 (H2BK120ub) a significant post-translational modification influencing transcriptional regulation. The modification aids in recruiting downstream effectors and is important for gene expression regulation and DNA damage repair mechanisms.

Pathways

RNF20 interacts with several cellular processes including the DNA damage response and transcriptional activation pathways. It significantly contributes to the DNA repair pathway by facilitating recruitment of repair proteins and is also involved in transcription regulation through the histone modification pathway. Furthermore RNF20 shows a functional relationship with RNF40 another E3 ligase in these pathways emphasizing its integrative role in cellular functioning.

Disruptions in RNF20 activity have connections to cancer and neurodegenerative disorders. Altered RNF20 expression or mutations can influence tumor growth due to impaired regulation of gene expression and DNA repair impacting proteins like p53. Additionally in neurodegenerative disorders anomalies in RNF20-mediated ubiquitination can affect protein homeostasis linking to proteins involved in neuronal integrity and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.. (Microbial infection) Promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing the expression of lytic viral genes including the latency switch gene RTA/ORF50.
See full target information RNF20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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