AB250142

Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RNF21/IFP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IFP1, RNF21, TRIM34, E3 ubiquitin-protein ligase TRIM34, Interferon-responsive finger protein 1, RING finger protein 21

3 Images

Flow Cytometry (Intracellular) - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (AB250142)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (AB250142)

This data was developed using ab180130, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of K562 cells labeling RNF21/IFP1 using ab180130 at 1/60 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control : Rabbit IgG.

Supplier Data

Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (AB250142)

This data was developed using ab180130, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] (<a href='/en-us/products/primary-antibodies/rnf21-ifp1-antibody-epr113052-ab180130'>ab180130</a>) at 1/10000 dilution

All lanes:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

false

Supplier Data

Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (AB250142)

This data was developed using ab180130, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] (<a href='/en-us/products/primary-antibodies/rnf21-ifp1-antibody-epr113052-ab180130'>ab180130</a>) at 1/2000 dilution

All lanes:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 57 kDa

false

Unconjugated

Anti-RNF21/IFP1 antibody [EPR11305(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11305(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }

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Product details

ab250142 is the carrier-free version of ab180130.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF21 also known as IFP1 is a human protein with a molecular weight of approximately 43 kDa. It belongs to the RING finger protein family functioning as an E3 ubiquitin ligase. Members of this family typically regulate protein degradation through the ubiquitin-proteasome system. RNF21 is widely expressed in various tissues including the brain liver and heart. This expression suggests important roles across different physiological contexts.

Biological function summary

The E3 ubiquitin ligase activity of RNF21 contributes to the regulation of protein homeostasis. RNF21 potentially participates in forming larger protein complexes which could be involved in cellular processes like cell cycle regulation. By tagging specific proteins for degradation RNF21 maintains cellular protein equilibrium. This process impacts various cellular functions including signal transduction and cellular stress responses.

Pathways

The function of RNF21 integrates within the ubiquitin-proteasome pathway and connects to protein turnover. This pathway involves numerous proteins such as ubiquitin-conjugating enzymes which work alongside RNF21. Additionally RNF21 may intersect with signaling pathways related to protein stability where it collaborates with proteins like SCF complexes which also function in protein degradation.

RNF21 has connections to neurodegenerative diseases such as Alzheimer's disease where protein aggregation becomes problematic. Aberrant protein degradation can exacerbate these disease conditions. RNF21's interactions with proteins like tau which is involved in Alzheimer's pathology illustrate its potential influence. Moreover its role in inflammatory responses might link RNF21 to disorders like rheumatoid arthritis where improper protein regulation plays a role.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as antiviral protein and contributes to the defense against retroviral infections (PubMed : 17156811, PubMed : 32282853). Acts as a capsid-specific restriction factor with the help of TRIM5 and prevents infection from non-host-adapted retroviruses (PubMed : 32282853). During influenza A virus infection, promotes programmed cell death by targeting ZBP1 for 'Lys-63'-linked polyubiquitination (PubMed : 35065966). In turn, promotes ZBP1 recruitment of RIPK3 to mediate virus-induced programmed necrosis (PubMed : 35065966). Negatively regulates the function of mitochondria by enhancing mitochondrial depolarization leading to cytochrome c release and mitochondria-dependent apoptosis (PubMed : 31956709). Promotes also the formation of multinucleated giant cells by means of cell fusion and phagocytosis in epithelial cells (PubMed : 31487507).

See full target information TRIM34

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (AB250142)

Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (AB250142)

Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (AB250142)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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