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AB248993

Anti-RNF212 antibody [EPR8796] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RNF212 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Probable E3 SUMO-protein ligase RNF212, Probable E3 SUMO-protein transferase RNF212, RING finger protein 212, RNF212

1 Images
Western blot - Anti-RNF212 antibody [EPR8796] - BSA and Azide free (AB248993)
  • WB

Unknown

Western blot - Anti-RNF212 antibody [EPR8796] - BSA and Azide free (AB248993)

This data was developed using ab151540, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNF212 antibody [EPR8796] (<a href='/en-us/products/primary-antibodies/rnf212-antibody-epr8796-ab151540'>ab151540</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8796

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248993 is the carrier-free version of ab151540.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF212 also known as Ring Finger Protein 212 is an E3 ubiquitin ligase with a mass of approximately 79 kDa. This protein mainly functions by facilitating the ubiquitination process which is critical for tagging proteins for degradation regulating their location and controlling their activity. RNF212 is expressed in germ cells particularly during meiosis where it plays a role in crossover formation and stabilization. Its activity is mostly seen within the context of cellular mechanisms related to reproductive biology.
Biological function summary

The role of RNF212 includes the promotion of meiotic recombination and ensuring proper chromosome segregation. It acts as a part of the large multiprotein complex that includes components like ZMM proteins. RNF212 is essential for crossover formation during meiosis acting as a stabilizer that ensures meiotic recombination reaches completion. This action is important for the proper maintenance of genomic integrity during gametogenesis.

Pathways

RNF212 is integral to the meiosis-specific processes and genetic recombination pathways. It plays an essential role in the homologous recombination pathway ensuring accurate DNA repair and recombination events. RNF212 interacts with protein partners such as HEI10 and MER3 within these pathways. The activity of RNF212 within these pathways ensures the genetic diversity necessary for evolutionary processes and proper genetic inheritance.

RNF212 has been linked to disorders such as infertility and certain aneuploidies. Its role in the regulation of meiotic recombination and chromosomal segregation connects it to problems arising from improper crossover events. Deficiencies or mutations in RNF212 can result in meiotic failures leading to infertility or developmental disorders. Within this context interactions with proteins like SYCP1 and MLH1 become relevant as these proteins are also implicated in ensuring proper meiotic function and genetic stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SUMO E3 ligase that acts as a regulator of crossing-over during meiosis : required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May mediate sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement and plays an antagonist role with CCNB1IP1/HEI10 in the regulation of meiotic recombination (By similarity).
See full target information RNF212
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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