JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB180850

Anti-RNF214 antibody [EPR12940]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal RNF214 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RING finger protein 214, RNF214

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] (AB180850)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNF214 antibody [EPR12940] (AB180850)

Immunofluorescence analysis of HepG2 cells labeling RNF214 using ab180850 at 1/50 dilution.

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (AB180850)
  • IP

Supplier Data

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (AB180850)

Western blot analysis on immunoprecipitation pellet from HT-1376 cell lysate labeling RBF214 using ab180850 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-RNF214 antibody [EPR12940] (ab180850)

Predicted band size: 78 kDa

false

Western blot - Anti-RNF214 antibody [EPR12940] (AB180850)
  • WB

Supplier Data

Western blot - Anti-RNF214 antibody [EPR12940] (AB180850)

All lanes:

Western blot - Anti-RNF214 antibody [EPR12940] (ab180850) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 10 µg

Lane 2:

HT-1376 lysates at 10 µg

Lane 3:

HepG2 lysates at 10 µg

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12940

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF214 also known as Ring Finger Protein 214 is an E3 ubiquitin-protein ligase. It plays a role in tagging proteins with ubiquitin for regulated protein degradation. The protein has a mass of approximately 74 kDa. RNF214 shows expression in many tissues including prostate brain and ovary. This wide expression pattern implies a diverse function potential in cellular processes.
Biological function summary

RNF214 participates in protein homeostasis by facilitating the ubiquitination process. It functions as part of an E3 ligase complex which delivers ubiquitin molecules to substrate proteins. This modification signals these proteins for degradation by the proteasome. Through this activity RNF214 influences protein turnover and maintains cell homeostasis by removing damaged or misfolded proteins ensuring functional protein networks.

Pathways

RNF214 is involved in the ubiquitin-proteasome system and the regulation of MAPK signaling pathways. The protein works closely with other ubiquitin ligases to control protein levels impacting cellular responses like cell cycle and growth. In the MAPK signaling pathway RNF214 can modulate the stability of signaling molecules like MAPK which impacts cell proliferation and differentiation.

RNF214 has associations with neurodegenerative diseases and cancer. Aberrant functioning of RNF214 could lead to an accumulation of misfolded proteins contributing to neurodegenerative conditions like Alzheimer's disease. In cancer changes in RNF214 activity might disrupt normal cell cycle control leading to tumorigenesis. It often connects with other proteins like p53 in the context of these diseases highlighting the importance of its regulatory role in cellular homeostasis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information RNF214
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:29823-29832 PubMed28415735

2017

Salicylate •Phenanthroline copper (II) complex induces apoptosis in triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Limei Fan,Muyou Tian,Yunyi Liu,Ying Deng,Zhengkai Liao,Jinhua Xu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB220163

Anti-YTHDF2 antibody [EPR20318]

RabMAb|Recombinant|KO Validated

Western blot - Anti-YTHDF2 antibody [EPR20318] (AB220163)

  • 5
  • 3
Primary Antibodies

AB267377

Anti-Wilms Tumor Protein antibody [EPR23963-116]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] (AB267377)

  • 0
  • 0
Primary Antibodies

AB273052

Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free (AB273052)

  • 0
  • 0
Primary Antibodies

AB281094

Anti-Wnt16 antibody [EPR22889-129] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Wnt16 antibody [EPR22889-129] - BSA and Azide free (Detector) (AB281094)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com