AB226215

Anti-RNF216 antibody

Name: Anti-RNF216 antibody (ab226215) | Abcam
Brand: Abcam Limited
SKU: AB226215
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RNF216 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNF216 aa 100-150.

View Alternative Names

TRIAD3, UBCE7IP1, ZIN, RNF216, E3 ubiquitin-protein ligase RNF216, RING finger protein 216, RING-type E3 ubiquitin transferase RNF216, Triad domain-containing protein 3, Ubiquitin-conjugating enzyme 7-interacting protein 1, Zinc finger protein inhibiting NF-kappa-B

2 Images

Supplier Data

Immunoprecipitation - Anti-RNF216 antibody (AB226215)

RNF216 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226215 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226215 at 1 μg/ml.

Lane 1 : ab226215 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RNF216 antibody (ab226215)

Predicted band size: 99 kDa

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Supplier Data

Western blot - Anti-RNF216 antibody (AB226215)

All lanes:

Western blot - Anti-RNF216 antibody (ab226215) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Predicted band size: 99 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RNF216 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q9NWF9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226215 was affinity purified using an epitope specific to RNF216 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The molecular structure RNF216 also known as Ring Finger Protein 216 and TRIAD3A is an E3 ubiquitin-protein ligase. RNF216 weighs approximately 101 kDa and contains a RING finger domain which is important for its ligase activity. This protein labels specific target proteins with ubiquitin marking them for degradation by the proteasome. RNF216 can be found in various tissues in the body indicating a widespread expression but it is significantly present in neural tissues.

Biological function summary

RNF216 functions in protein homeostasis and immune system regulation. It acts as a mediator in the disposal of misfolded proteins preventing accumulation that could lead to cellular stress. RNF216 does not work alone; it often interacts with E2 ubiquitin-conjugating enzymes to facilitate substrate targeting and ubiquitination. Furthermore RNF216 is essential for innate immune response modulation through degradation of immune stimulatory proteins.

Pathways

RNF216 takes part in the protein degradation pathway and immune signaling pathways. In the ubiquitin-proteasome system RNF216 collaborates with E2 enzymes as an important player in proteolysis. It also plays a role in regulating the NF-kB pathway by affecting the levels of proteins necessary for NF-kB activation such as TRAF3. These interactions ensure that response to stress and infections is tightly controlled which otherwise could lead to inflammatory conditions.

RNF216 is linked to neurological disorders and immune system dysregulation. Mutations or dysregulation of RNF216 can contribute to Gordon Holmes syndrome which involves movement and endocrine problems due to neural dysfunction. RNF216 also affects immune-related conditions by interacting with proteins like TRAF6 which play a role in immune signaling. Understanding RNF216's role provides insights into potential therapeutic targets for managing such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Isoform 1. E3 ubiquitin ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their ubiquitination (PubMed : 34998453). Plays a role in the regulation of antiviral responses by promoting the degradation of TRAF3, TLR4 and TLR9 (PubMed : 15107846, PubMed : 19893624). In turn, down-regulates NF-kappa-B and IRF3 activation as well as beta interferon production. Participates also in the regulation of autophagy by ubiquitinating BECN1 leading to its degradation and autophagy inhibition (PubMed : 25484083). Plays a role in ARC-dependent synaptic plasticity by mediating ARC ubiquitination resulting in its rapid proteasomal degradation (PubMed : 24945773). Plays aso an essential role in spermatogenesis and male fertility (By similarity). Mechanistically, regulates meiosis by promoting the degradation of PRKACB through the ubiquitin-mediated lysosome pathway (By similarity). Modulates the gonadotropin-releasing hormone signal pathway by affecting the stability of STAU2 that is required for the microtubule-dependent transport of neuronal RNA from the cell body to the dendrite (By similarity).. Isoform 3. Inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis.

See full target information RNF216

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American Society of Gene Therapy 31:2524-2542 PubMed37340635

2023

Smoking and tetramer tryptase accelerate intervertebral disc degeneration by inducing METTL14-mediated DIXDC1 m modification.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Tu,Wentian Li,Philip M Hansbro,Qi Yan,Xupeng Bai,Chantal Donovan,Richard Y Kim,Izabela Galvao,Abhirup Das,Cao Yang,Jun Zou,Ashish Diwan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RNF216 antibody

Immunoprecipitation - Anti-RNF216 antibody (AB226215)

Western blot - Anti-RNF216 antibody (AB226215)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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