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AB25961

Anti-RNF216 antibody

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(5 Publications)

Rabbit Polyclonal RNF216 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Rnf216.

View Alternative Names

Triad3, Ubce7ip1, Uip83, Zin, E3 ubiquitin-protein ligase RNF216, RING finger protein 216, RING-type E3 ubiquitin transferase RNF216, Triad domain-containing protein 3, UbcM4-interacting protein 83, Ubiquitin-conjugating enzyme 7-interacting protein 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RNF216 antibody (AB25961)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RNF216 antibody (AB25961)

Immunofluorescence of TRIAD3A in Mouse Heart cells using ab25961 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF216 antibody (AB25961)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF216 antibody (AB25961)

ab25961 at 10μg/ml staining RNF216 in mouse heart by IHC

Western blot - Anti-RNF216 antibody (AB25961)
  • WB

Unknown

Western blot - Anti-RNF216 antibody (AB25961)

Lane 1:

Western blot - Anti-RNF216 antibody (ab25961) at 0.5 µg/mL

Lane 2:

Western blot - Anti-RNF216 antibody (ab25961) at 1 µg/mL

Lane 3:

Western blot - Anti-RNF216 antibody (ab25961) at 2 µg/mL

All lanes:

Mouse heart cell lysates

Predicted band size: 99 kDa

Observed band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Rnf216. The exact immunogen used to generate this antibody is proprietary information.

P58283

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by ion exchange chromatography
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The molecular structure RNF216 also known as Ring Finger Protein 216 and TRIAD3A is an E3 ubiquitin-protein ligase. RNF216 weighs approximately 101 kDa and contains a RING finger domain which is important for its ligase activity. This protein labels specific target proteins with ubiquitin marking them for degradation by the proteasome. RNF216 can be found in various tissues in the body indicating a widespread expression but it is significantly present in neural tissues.
Biological function summary

RNF216 functions in protein homeostasis and immune system regulation. It acts as a mediator in the disposal of misfolded proteins preventing accumulation that could lead to cellular stress. RNF216 does not work alone; it often interacts with E2 ubiquitin-conjugating enzymes to facilitate substrate targeting and ubiquitination. Furthermore RNF216 is essential for innate immune response modulation through degradation of immune stimulatory proteins.

Pathways

RNF216 takes part in the protein degradation pathway and immune signaling pathways. In the ubiquitin-proteasome system RNF216 collaborates with E2 enzymes as an important player in proteolysis. It also plays a role in regulating the NF-kB pathway by affecting the levels of proteins necessary for NF-kB activation such as TRAF3. These interactions ensure that response to stress and infections is tightly controlled which otherwise could lead to inflammatory conditions.

RNF216 is linked to neurological disorders and immune system dysregulation. Mutations or dysregulation of RNF216 can contribute to Gordon Holmes syndrome which involves movement and endocrine problems due to neural dysfunction. RNF216 also affects immune-related conditions by interacting with proteins like TRAF6 which play a role in immune signaling. Understanding RNF216's role provides insights into potential therapeutic targets for managing such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their ubiquitination. Plays a role in the regulation of antiviral responses by promoting the degradation of TRAF3, TLR4 and TLR9. In turn, down-regulates NF-kappa-B and IRF3 activation as well as beta interferon production. Participates also in the regulation of autophagy by ubiquitinating BECN1 leading to its degradation and autophagy inhibition. Plays a role in ARC-dependent synaptic plasticity by mediating ARC ubiquitination resulting in its rapid proteasomal degradation (By similarity). Plays aso an essential role in spermatogenesis and male fertility (PubMed : 30649198). Mechanistically, regulates meiosis by promoting the degradation of PRKACB through the ubiquitin-mediated lysosome pathway (PubMed : 33724554). Modulates the gonadotropin-releasing hormone signal pathway by affecting the stability of STAU2 that is required for the microtubule-dependent transport of neuronal RNA from the cell body to the dendrite (PubMed : 37439148).
See full target information Rnf216
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 11:e925 PubMed37506157

2023

Triad3A involved in the regulation of endotoxin tolerance and mycobactericidal activity through the NFκB-nitric oxide pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongwei Qin,Jinliang Chen,Kuang Xu,Yang Lu,Feifan Xu,Jiahai Shi

American journal of respiratory cell and molecular biology 62:157-167 PubMed31385713

2019

Toll-like Receptor 8 Stability Is Regulated by Ring Finger 216 in Response to Circulating MicroRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

John Evankovich,Travis Lear,Courtney Baldwin,Yanwen Chen,Virginia White,John Villandre,James Londino,Yuan Liu,Bryan McVerry,Georgios D Kitsios,Rama K Mallampalli,Bill B Chen

Autophagy 10:2239-50 PubMed25484083

2014

Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Congfeng Xu,Kuan Feng,Xiaonan Zhao,Shiqian Huang,Yiji Cheng,Liu Qian,Yanan Wang,Hongxing Sun,Min Jin,Tsung-Hsien Chuang,Yanyun Zhang

Journal of immunology (Baltimore, Md. : 1950) 192:714-21 PubMed24337384

2013

TNFR-associated factor 6 and TGF-β-activated kinase 1 control signals for a senescence response by an endosomal NK cell receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Sumati Rajagopalan,Elizabeth C Lee,Matthew L DuPrie,Eric O Long

Journal of immunology (Baltimore, Md. : 1950) 186:2959-69 PubMed21270397

2011

Ubiquitylation of an internalized killer cell Ig-like receptor by Triad3A disrupts sustained NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

S M Shahjahan Miah,Amanda K Purdy,Nicholas B Rodin,Alexander W MacFarlane,Jennifer Oshinsky,Diana A Alvarez-Arias,Kerry S Campbell
View all publications
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