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AB140514

Anti-RNF25 antibody

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Rabbit Polyclonal RNF25 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human E3 ubiquitin-protein ligase RNF25 aa 400 to C-terminus.

View Alternative Names

E3 ubiquitin-protein ligase RNF25, RING finger protein 25, RING finger protein AO7, RNF25

2 Images
Western blot - Anti-RNF25 antibody (AB140514)
  • WB

Unknown

Western blot - Anti-RNF25 antibody (AB140514)

All lanes:

Western blot - Anti-RNF25 antibody (ab140514) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 51 kDa

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Exposure time: 30s

Immunoprecipitation - Anti-RNF25 antibody (AB140514)
  • IP

Unknown

Immunoprecipitation - Anti-RNF25 antibody (AB140514)

Immunoprecipitation of RNF25 from HeLa whole cell lysate using ab140514 at 6 µg/mg lysate (1 mg of lysate for IP; 20% of IP loaded).
For blotting immunoprecipitated RNF25, ab140514 was used at 1 µg/ml.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RNF25 antibody (ab140514)

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human E3 ubiquitin-protein ligase RNF25 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96BH1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab140514 was affinity purified using an epitope specific to RNF25 immobilized on a solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF25 also known as RING Finger Protein 25 is a protein that acts like an E3 ubiquitin ligase. This protein has a molecular mass of approximately 40 kDa. RNF25 is involved in tagging proteins with ubiquitin a process known as ubiquitination which directs these proteins for degradation by the proteasome. You will find RNF25 expressed in a variety of human tissues with higher levels observed in the heart and skeletal muscle.
Biological function summary

The function of RNF25 impacts protein turnover and stability. It often associates with other proteins to form complexes that regulate important cellular processes such as protein homeostasis and response to stress. RNF25 contributes to the maintenance of cellular equilibrium by marking defective or unneeded proteins for removal.

Pathways

RNF25 engages in the ubiquitin-proteasome pathway which oversees the controlled degradation of proteins. This pathway is central to many cellular processes including cell cycle regulation and signal transduction. RNF25 also interacts with proteins like NF-kB playing a role in the NF-kB signaling pathway which is important in inflammatory responses.

RNF25 associations are evident in contexts like cancer and immune system disorders. Dysregulation of ubiquitination involving RNF25 can contribute to oncogenesis and affect tumor suppressor proteins such as p53. Similarly alterations in the NF-kB pathway with links to RNF25 have been implicated in autoimmune diseases indicating its significance in the regulation of immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes (PubMed : 36638793, PubMed : 37651229, PubMed : 37951216). Catalyzes ubiquitination of RPS27A in response to ribosome collisions, promoting activation of RNF14 (PubMed : 36638793). RNF25 catalyzes ubiquitination of other ribosomal proteins on stalled ribosomes, such as RPL0, RPL1, RPL12, RPS13 and RPS17 (PubMed : 36638793). Also involved in ubiquitination and degradation of stalled ETF1/eRF1 (PubMed : 36638793, PubMed : 37651229). Independently of its function in the response to stalled ribosomes, mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). May also stimulate transcription mediated by NF-kappa-B via its interaction with RELA/p65 (PubMed : 12748188).
See full target information E3 ubiquitin-protein ligase RNF25
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Product promise

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