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AB89281

Anti-RNF25 antibody

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(3 Publications )

Mouse Polyclonal RNF25 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human E3 ubiquitin-protein ligase RNF25.
2 Images
Western blot - Anti-RNF25 antibody (AB89281)
  • WB

Unknown

Western blot - Anti-RNF25 antibody (AB89281)

All lanes:

Western blot - Anti-RNF25 antibody (ab89281) at 1 µg/mL

All lanes:

HeLa Nuclear extract at 50 µg

Predicted band size: 51 kDa

Observed band size: 60 kDa

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Western blot - Anti-RNF25 antibody (AB89281)
  • WB

Unknown

Western blot - Anti-RNF25 antibody (AB89281)

All lanes:

Western blot - Anti-RNF25 antibody (ab89281) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 51 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human E3 ubiquitin-protein ligase RNF25.

Q96BH1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF25 also known as RING Finger Protein 25 is a protein that acts like an E3 ubiquitin ligase. This protein has a molecular mass of approximately 40 kDa. RNF25 is involved in tagging proteins with ubiquitin a process known as ubiquitination which directs these proteins for degradation by the proteasome. You will find RNF25 expressed in a variety of human tissues with higher levels observed in the heart and skeletal muscle.
Biological function summary

The function of RNF25 impacts protein turnover and stability. It often associates with other proteins to form complexes that regulate important cellular processes such as protein homeostasis and response to stress. RNF25 contributes to the maintenance of cellular equilibrium by marking defective or unneeded proteins for removal.

Pathways

RNF25 engages in the ubiquitin-proteasome pathway which oversees the controlled degradation of proteins. This pathway is central to many cellular processes including cell cycle regulation and signal transduction. RNF25 also interacts with proteins like NF-kB playing a role in the NF-kB signaling pathway which is important in inflammatory responses.

RNF25 associations are evident in contexts like cancer and immune system disorders. Dysregulation of ubiquitination involving RNF25 can contribute to oncogenesis and affect tumor suppressor proteins such as p53. Similarly alterations in the NF-kB pathway with links to RNF25 have been implicated in autoimmune diseases indicating its significance in the regulation of immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that plays a key role in the RNF14-RNF25 translation quality control pathway, a pathway that takes place when a ribosome has stalled during translation, and which promotes ubiquitination and degradation of translation factors on stalled ribosomes (PubMed : 36638793, PubMed : 37651229, PubMed : 37951216). Catalyzes ubiquitination of RPS27A in response to ribosome collisions, promoting activation of RNF14 (PubMed : 36638793). RNF25 catalyzes ubiquitination of other ribosomal proteins on stalled ribosomes, such as RPL0, RPL1, RPL12, RPS13 and RPS17 (PubMed : 36638793). Also involved in ubiquitination and degradation of stalled ETF1/eRF1 (PubMed : 36638793, PubMed : 37651229). Independently of its function in the response to stalled ribosomes, mediates ubiquitination and subsequent proteasomal degradation of NKD2 (By similarity). May also stimulate transcription mediated by NF-kappa-B via its interaction with RELA/p65 (PubMed : 12748188).
See full target information E3 ubiquitin-protein ligase RNF25

Alternative Names

E3 ubiquitin-protein ligase RNF25, RING finger protein 25, RING finger protein AO7, RNF25
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306929 PubMed38286671

2024

Oxidative Stress Promotes Liver Cancer Metastasis via RNF25-Mediated E-Cadherin Protein Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Huang,Li Zhou,Jiufei Duan,Siyuan Qin,Jingwen Jiang,Haining Chen,Kui Wang,Rui Liu,Minlan Yuan,Xiangdong Tang,Edouard C Nice,Yuquan Wei,Wei Zhang,Canhua Huang

Molecular cell 83:4290-4303.e9 PubMed37951216

2023

RNF14-dependent atypical ubiquitylation promotes translation-coupled resolution of RNA-protein crosslinks.

Applications

Unspecified application

Species

Unspecified reactive species

Shubo Zhao,Jacqueline Cordes,Karolina M Caban,Maximilian J Götz,Timur Mackens-Kiani,Anthony J Veltri,Niladri K Sinha,Pedro Weickert,Selay Kaya,Graeme Hewitt,Danny D Nedialkova,Thomas Fröhlich,Roland Beckmann,Allen R Buskirk,Rachel Green,Julian Stingele

Cell death & disease 9:587 PubMed29789542

2018

RNF25 promotes gefitinib resistance in EGFR-mutant NSCLC cells by inducing NF-κB-mediated ERK reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Hee Cho,Yeon-Mi You,Y I Yeom,Dong Chul Lee,Bo-Kyung Kim,Misun Won,Byoung Chul Cho,Minho Kang,Seulki Park,Suk-Jin Yang,Jang Seong Kim,Jung-Ae Kim,Kyung Chan Park
View all publications
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Product promise

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