Knockout Tested Rabbit Polyclonal RNF31/HOIP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human RNF31 aa 1000 to C-terminus.
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- Disease models & mechanisms 16:2023An interaction between OTULIN and SCRIB uncovers roles for linear ubiquitination in planar cell polarity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie M Almeida,Sofiia Ivantsiv,Rieko Niibori,Wade H Dunham,Brooke A Green,Liang Zhao,Anne-Claude Gingras,Sabine P CordesPubMed 37589075
- The FEBS journal 290:4224-42372023Linear ubiquitination improves NFAT1 protein stability and facilitates NFAT1 signalling in Kawasaki disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Miao,Guanghui Qian,Renxia Zhang,Yukang Yuan,Yibo Zuo,Yueyue Ding,Xuan Li,Yunjia Tang,Hui Zheng,Haitao LvPubMed 36779231
- Journal of experimental & clinical cancer research : CR 41:3642022RNF31 represses cell progression and immune evasion via YAP/PD-L1 suppression in triple negative breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuijie Yang,Min Xue,Peng Su,Yan Zhou,Xin Li,Zhongbo Li,Yan Xia,Chenmiao Zhang,Mingxi Fu,Xiuxia Zheng,Guosheng Luo,Tian Wei,Xinxing Wang,Yinlu Ding,Jian Zhu,Ting ZhuangPubMed 36581998
- Oncology letters 24:3942022Identification and molecular analysis of RNF31 Q622H germline polymorphism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeshiru Nakazawa,Ryo Mamiya,Reika Kawabata-Iwakawa,Daisuke Oikawa,Kyoichi Kaira,Fuminori Tokunaga,Sumihito Nobusawa,Yusuke Sato,Atsushi Sasaki,Toshiki Yajima,Ken ShirabePubMed 36276481
- Cells 11:2022Suppression of Linear Ubiquitination Ameliorates Cytoplasmic Aggregation of Truncated TDP-43.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiang Zhang,Seigo Terawaki,Daisuke Oikawa,Yoshinori Okina,Yoshinosuke Usuki,Hidefumi Ito,Fuminori TokunagaPubMed 35954242
- Frontiers in immunology 12:6354752021Crosstalk Between NDP52 and LUBAC in Innate Immune Responses, Cell Death, and Xenophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHirohisa Miyashita,Daisuke Oikawa,Seigo Terawaki,Daijiro Kabata,Ayumi Shintani,Fuminori TokunagaPubMed 33815386
- Frontiers in immunology 11:6019262020Cellular and Mathematical Analyses of LUBAC Involvement in T Cell Receptor-Mediated NF-κB Activation Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaisuke Oikawa et. al.PubMed 33329596
- Communications biology 3:1632020Molecular bases for HOIPINs-mediated inhibition of LUBAC and innate immune responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaisuke Oikawa et. al.PubMed 32246052
- Nature communications 11:11462020Regulation of the linear ubiquitination of STAT1 controls antiviral interferon signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYibo Zuo et. al.PubMed 32123171
- Molecular cell 54:349-612014Binding of OTULIN to the PUB domain of HOIP controls NF-κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronique Schaeffer et. al.PubMed 24726327
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RNF31 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96EP0
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant | Dilution info - | Notes - |
Associated Products
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Target data
Function
E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:17006537, PubMed:19136968, PubMed:20005846, PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22863777, PubMed:28189684, PubMed:28481331). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (PubMed:17006537, PubMed:19136968, PubMed:20005846, PubMed:21455173, PubMed:21455180, PubMed:21455181, PubMed:22863777, PubMed:28189684). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed:21455173, PubMed:28189684). LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (PubMed:20005846, PubMed:27458237). The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria (PubMed:28481331, PubMed:34012115). LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin (PubMed:28481331). Recruited to the surface of bacteria by RNF213, which initiates the bacterial ubiquitin coat (PubMed:34012115). The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed:28481331, PubMed:34012115). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (PubMed:23708998). RNF31 is required for linear ubiquitination of BCL10, thereby promoting TCR-induced NF-kappa-B activation (PubMed:27777308). Binds polyubiquitin of different linkage types (PubMed:23708998).
Alternative names
ZIBRA, RNF31, E3 ubiquitin-protein ligase RNF31, HOIL-1-interacting protein, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, Zinc in-between-RING-finger ubiquitin-associated domain protein, HOIP
Recommended products
Knockout Tested Rabbit Polyclonal RNF31/HOIP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human RNF31 aa 1000 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RNF31 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96EP0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RNF31 also known as HOIP (HOIL-1 Interacting Protein) is a large protein with a molecular weight of around 119 kDa. It is a part of the ubiquitin-proteasome system and functions mechanically as a E3 ubiquitin ligase. RNF31 forms the catalytic component of the Linear Ubiquitin Chain Assembly Complex (LUBAC). Expression of RNF31 occurs in many tissues with notable levels in immune cells and the testes. The protein features a RING-in-between-RING (RBR) motif structure that is critical for its ligase activity.
- Biological function summary
RNF31/HOIP plays an important role in immune response regulation. It functions as part of the LUBAC complex alongside SHARPIN and RBCK1 (also known as HOIL-1L) which together catalyze the linear ubiquitination of substrates. These modifications regulate NF-kB signaling which is an important pathway involved in immune and inflammatory responses. RNF31's ubiquitin ligase activity adds linear ubiquitin chains to target proteins influencing their stability and function therefore impacting the immune cell function and survival.
- Pathways
RNF31/HOIP integrates into the NF-kB and the apoptosis pathways. In the NF-kB pathway it acts as a regulator of cytokine production and cell survival. RNF31 achieves this through modification of NEMO (NF-kB essential modulator) an important protein in this pathway. In apoptosis regulation linear ubiquitination by RNF31 modulates the activity of proteins such as caspases preventing inappropriate cell death during immune responses. This helps to maintain balance between survival and death signals in cells allowing for proper immune function.
- Associated diseases and disorders
RNF31/HOIP has implications in inflammatory diseases and certain cancers. Dysregulation of LUBAC components including HOIP has been linked with chronic inflammatory conditions like rheumatoid arthritis where inappropriate NF-kB activation occurs. Furthermore aberrations in RNF31 activity have associations with tumorigenesis where its altered signaling pathways can lead to uncontrolled cell proliferation. In these contexts proteins such as A20 may interact with RNF31 affecting the regulation of NF-kB signaling therefore influencing disease progression.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunoprecipitation - Anti-RNF31/HOIP antibody (ab125189)
Detection of human RNF31/HOIP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF31/HOIP antibody ab125189 used for IP at 6 μg per reaction. RNF31/HOIP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated RNF31/HOIP, ab125189 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-RNF31/HOIP antibody (ab125189)
Predicted band size: 120 kDa
Western blot - Anti-RNF31/HOIP antibody (ab125189)
All lanes: Western blot - Anti-RNF31/HOIP antibody (ab125189) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 3min
Western blot - Anti-RNF31/HOIP antibody (ab125189)
Western blot: Anti-RNF31 antibody (ab125189) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab125189 was shown to bind specifically to RNF31. A band was observed at 130 kDa in wild-type A549 cell lysates with no signal observed at this size in RNF31 knockout cell line. To generate this image, wild-type and RNF31 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-RNF31/HOIP antibody (ab125189) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: RNF31 knockout A549 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 130 kDa
Western blot - Anti-RNF31/HOIP antibody (ab125189)
Western blot: Anti-RNF31 antibody (ab125189) staining at 1/3000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab125189 was shown to bind specifically to RNF31. A band was observed at 130-140 kDa in wild-type A549 cell lysates with no signal observed at this size in RNF31 knockout cell line. To generate this image, wild-type and RNF31 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-RNF31/HOIP antibody (ab125189) at 1/3000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: RNF31 knockout A549 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
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