AB316244

Anti-RNF31/HOIP antibody [EPR27947-3] - BSA and Azide free (Capture)

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal RNF31/HOIP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

ZIBRA, RNF31, E3 ubiquitin-protein ligase RNF31, HOIL-1-interacting protein, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, Zinc in-between-RING-finger ubiquitin-associated domain protein, HOIP

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Sandwich ELISA - Anti-RNF31/HOIP antibody [EPR27947-3] - BSA and Azide free (Capture) (AB316244)

sELISA

Supplier Data

Sandwich ELISA - Anti-RNF31/HOIP antibody [EPR27947-3] - BSA and Azide free (Capture) (AB316244)

Sandwich ELISA of ab316243 with the capture antibody ab316244 dilution at 2 ug/mL and detector antibody ab316245 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27947-3

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab316244 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RNF31/HOIP. The recommended pair for sandwich ELISA is:

Capture: ab316244, Human RNF31/HOIP Capture Antibody (unconjugated)

Detector: ab316245, Human RNF31/HOIP Detector Antibody (unconjugated)

The reference range value is 0.16-10 ng/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF31 also known as HOIP (HOIL-1 Interacting Protein) is a large protein with a molecular weight of around 119 kDa. It is a part of the ubiquitin-proteasome system and functions mechanically as a E3 ubiquitin ligase. RNF31 forms the catalytic component of the Linear Ubiquitin Chain Assembly Complex (LUBAC). Expression of RNF31 occurs in many tissues with notable levels in immune cells and the testes. The protein features a RING-in-between-RING (RBR) motif structure that is critical for its ligase activity.

Biological function summary

RNF31/HOIP plays an important role in immune response regulation. It functions as part of the LUBAC complex alongside SHARPIN and RBCK1 (also known as HOIL-1L) which together catalyze the linear ubiquitination of substrates. These modifications regulate NF-kB signaling which is an important pathway involved in immune and inflammatory responses. RNF31's ubiquitin ligase activity adds linear ubiquitin chains to target proteins influencing their stability and function therefore impacting the immune cell function and survival.

Pathways

RNF31/HOIP integrates into the NF-kB and the apoptosis pathways. In the NF-kB pathway it acts as a regulator of cytokine production and cell survival. RNF31 achieves this through modification of NEMO (NF-kB essential modulator) an important protein in this pathway. In apoptosis regulation linear ubiquitination by RNF31 modulates the activity of proteins such as caspases preventing inappropriate cell death during immune responses. This helps to maintain balance between survival and death signals in cells allowing for proper immune function.

RNF31/HOIP has implications in inflammatory diseases and certain cancers. Dysregulation of LUBAC components including HOIP has been linked with chronic inflammatory conditions like rheumatoid arthritis where inappropriate NF-kB activation occurs. Furthermore aberrations in RNF31 activity have associations with tumorigenesis where its altered signaling pathways can lead to uncontrolled cell proliferation. In these contexts proteins such as A20 may interact with RNF31 affecting the regulation of NF-kB signaling therefore influencing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed : 17006537, PubMed : 19136968, PubMed : 20005846, PubMed : 21455173, PubMed : 21455180, PubMed : 21455181, PubMed : 22863777, PubMed : 28189684, PubMed : 28481331). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (PubMed : 17006537, PubMed : 19136968, PubMed : 20005846, PubMed : 21455173, PubMed : 21455180, PubMed : 21455181, PubMed : 22863777, PubMed : 28189684). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed : 21455173, PubMed : 28189684). LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (PubMed : 20005846, PubMed : 27458237). The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria (PubMed : 28481331, PubMed : 34012115). LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin (PubMed : 28481331). Recruited to the surface of bacteria by RNF213, which initiates the bacterial ubiquitin coat (PubMed : 34012115). The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation (PubMed : 28481331, PubMed : 34012115). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (PubMed : 23708998). RNF31 is required for linear ubiquitination of BCL10, thereby promoting TCR-induced NF-kappa-B activation (PubMed : 27777308). Binds polyubiquitin of different linkage types (PubMed : 23708998).

See full target information RNF31

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Anti-RNF31/HOIP antibody [EPR27947-3] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-RNF31/HOIP antibody [EPR27947-3] - BSA and Azide free (Capture) (AB316244)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise