Mouse Polyclonal RNF34 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RNF34.
Images
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human RNF34. Database link Q969K3
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis (PubMed:15069192). May mediate 'Lys-48'-linked polyubiquitination of RIPK1 and its subsequent proteasomal degradation thereby indirectly regulating the tumor necrosis factor-mediated signaling pathway (Ref.13). Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation (PubMed:17121812). Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN (PubMed:18382127). Mediates PPARGC1A proteasomal degradation probably through ubiquitination thereby indirectly regulating the metabolism of brown fat cells (PubMed:22064484). Possibly involved in innate immunity, through 'Lys-48'-linked polyubiquitination of NOD1 and its subsequent proteasomal degradation (PubMed:25012219).
Alternative names
E3 ubiquitin-protein ligase RNF34, Caspase regulator CARP1, Caspases-8 and -10-associated RING finger protein 1, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RING finger protein 34, RING finger protein RIFF, RING-type E3 ubiquitin transferase RNF34, CARP-1, hRFI, RNF34
Recommended products
Mouse Polyclonal RNF34 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RNF34.
Alternative names
E3 ubiquitin-protein ligase RNF34, Caspase regulator CARP1, Caspases-8 and -10-associated RING finger protein 1, FYVE-RING finger protein Momo, Human RING finger homologous to inhibitor of apoptosis protein, RING finger protein 34, RING finger protein RIFF, RING-type E3 ubiquitin transferase RNF34, CARP-1, hRFI, RNF34
Key facts
- Isotype
IgG
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 99% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human RNF34. Database link Q969K3
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-RNF34 antibody (ab169047)
Immunofluorescent analysis of HeLa cells labeling RNF34 with ab169047 at 10 µg/ml.
Western blot - Anti-RNF34 antibody (ab169047)
All lanes: Western blot - Anti-RNF34 antibody (ab169047) at 1 µg/mL
Lane 1: RNF34 transfected 293T cell lysate at 15 µL
Lane 2: non transfected 293T cell lysate at 15 µL
SecondaryAll lanes: Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Downloads
Product protocols
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