AB111943

Anti-RNF36 antibody [EPR4755]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal RNF36 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

RNF36, HSD-34, HSD34, TRIM69, E3 ubiquitin-protein ligase TRIM69, RFP-like domain-containing protein trimless, RING finger protein 36, RING-type E3 ubiquitin transferase TRIM69, Tripartite motif-containing protein 69

2 Images

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Western blot - Anti-RNF36 antibody [EPR4755] (AB111943)

All lanes:

Western blot - Anti-RNF36 antibody [EPR4755] (ab111943) at 1/1000 dilution

All lanes:

Human testis lysate at 10 µg

Predicted band size: 57 kDa

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Western blot - Anti-RNF36 antibody [EPR4755] (AB111943)

All lanes:

Western blot - Anti-RNF36 antibody [EPR4755] (ab111943) at 1/1000 dilution

All lanes:

Neuro-2a cell lysate at 10 µg

Predicted band size: 57 kDa

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Carrier free

Anti-RNF36 antibody [EPR4755] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4755

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab111943 detects at least 4 isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase that plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis indiana virus (PubMed : 23131556, PubMed : 30142214, PubMed : 31375575, PubMed : 31578292). Ubiquitinates viral proteins such as dengue virus NS3 thereby limiting infection (PubMed : 30844644). In addition, acts as a key mediator of type I interferon induced microtubule stabilization by directly associating to microtubules independently of its E3 ligase activity (PubMed : 36251989). Also plays a role in cataract formation together with TP53 (PubMed : 30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed : 30844644). Regulates centrosome dynamics and mitotic progression by ubiquitinating STK3/MST2; leading to its redistribution to the perinuclear cytoskeleton and subsequent phosphorylation by PLK1 (PubMed : 37739411).

See full target information TRIM69

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 14:200-218 PubMed36741265

2023

Tripartite motif containing 69 elicits ERK2-dependent EYA4 turnover to impart pancreatic tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Jia,Hui-Yan Li,Jue Wang,Xing Chen,Lu Lou,Yan-Yan Wei,Ying Wang,Shi-Jing Mo

Journal of virology 93: PubMed31375575

2019

TRIM69 Inhibits Vesicular Stomatitis Indiana Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Suzannah J Rihn,Muhamad Afiq Aziz,Douglas G Stewart,Joseph Hughes,Matthew L Turnbull,Mariana Varela,Elena Sugrue,Christie S Herd,Megan Stanifer,Steven P Sinkins,Massimo Palmarini,Sam J Wilson

Redox biology 22:101157 PubMed30844644

2019

TRIM69 inhibits cataractogenesis by negatively regulating p53.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfang Rong,Jun Rao,Dan Li,Qinghe Jing,Yi Lu,Yinghong Ji

PLoS pathogens 14:e1007287 PubMed30142214

2018

Interferon-stimulated TRIM69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3.

Applications

Unspecified application

Species

Unspecified reactive species

Kezhen Wang,Chunling Zou,Xiujuan Wang,Chenxiao Huang,Tingting Feng,Wen Pan,Qihan Wu,Penghua Wang,Jianfeng Dai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RNF36 antibody [EPR4755]

Western blot - Anti-RNF36 antibody [EPR4755] (AB111943)

Western blot - Anti-RNF36 antibody [EPR4755] (AB111943)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

Product promise