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AB230438

Anti-RNF4 antibody

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(1 Publication)

Rabbit Polyclonal RNF4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RNF4 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

SNURF, RES4-26, RNF4, E3 ubiquitin-protein ligase RNF4, RING finger protein 4, Small nuclear ring finger protein, Protein SNURF

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF4 antibody (AB230438)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF4 antibody (AB230438)

Formalin-fixed, paraffin-embedded human colon tissue stained for RNF4 using ab230438 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-RNF4 antibody (AB230438)
  • WB

Supplier Data

Western blot - Anti-RNF4 antibody (AB230438)

All lanes:

Western blot - Anti-RNF4 antibody (ab230438) at 1/1000 dilution

All lanes:

LCNCaP (human prostate cancer cell line) cell lysate at 35 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RNF4 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P78317

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Cell Lines & Lysates

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation (PubMed : 18408734, PubMed : 19307308, PubMed : 35013556). Regulates the degradation of several proteins including PML and the transcriptional activator PEA3 (PubMed : 18408734, PubMed : 19307308, PubMed : 20943951). Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation (PubMed : 20212317). Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 (PubMed : 19779455, PubMed : 20026589). Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation (PubMed : 12885770). Catalyzes ubiquitination of sumoylated PARP1 in response to PARP1 trapping to chromatin, leading to PARP1 removal from chromatin by VCP/p97 (PubMed : 35013556).
See full target information RNF4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308823 PubMed39287146

2024

VPS34 Governs Oocyte Developmental Competence by Regulating Mito/Autophagy: A Novel Insight into the Significance of RAB7 Activity and Its Subcellular Location.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Liu,Kehan Wang,Yuting Lin,Lu Wang,Xin Jin,Yuexin Qiu,Wenya Sun,Ling Zhang,Yan Sun,Xiaowei Dou,Shiming Luo,Youqiang Su,Qingyuan Sun,Wenpei Xiang,Feiyang Diao,Jing Li
View all publications
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Product promise

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