Rabbit Recombinant Monoclonal RNF40 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--western-blot-img50962.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058)")
![Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--western-blot-img110841.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058)")
![Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--western-blot-img145614.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25259.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058)")
![Immunoprecipitation - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunoprecipitation-img53063.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (AB251058)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Expected | Expected | Tested |
| Rat | Expected | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role in histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. (Microbial infection) Promotes the human herpesvirus 8 (KSHV) lytic cycle by inducing the expression of lytic viral genes including the latency switch gene RTA/ORF50.
Alternative names
BRE1B, KIAA0661, RNF40, E3 ubiquitin-protein ligase BRE1B, BRE1-B, 95 kDa retinoblastoma-associated protein, RING finger protein 40, RING-type E3 ubiquitin transferase BRE1B, RBP95
Recommended products
Rabbit Recombinant Monoclonal RNF40 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18511
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251058 is the carrier-free version of Anti-RNF40 antibody [EPR18511] ab191309.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--western-blot-img50962.jpg "Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)
This data was developed using Anti-RNF40 antibody [EPR18511] ab191309, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the MW shown by commercial antibodies from other suppliers.
All lanes: Western blot - Anti-RNF40 antibody [EPR18511] (Anti-RNF40 antibody [EPR18511] ab191309) at 1/1000 dilution
Lane 1: Human fetal liver lysate at 10 µg
Lane 2: Human fetal heart lysate at 10 µg
Lane 3: Human fetal kidney lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa
Exposure time: 15s
![Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--western-blot-img110841.jpg "Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)
This data was developed using Anti-RNF40 antibody [EPR18511] ab191309, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1-3: 30 seconds; Lane 4-6: 15 seconds.
The molecular weight observed is consistent with the MW shown by commercial antibodies from other suppliers.
All lanes: Western blot - Anti-RNF40 antibody [EPR18511] (Anti-RNF40 antibody [EPR18511] ab191309) at 1/1000 dilution
Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
Lane 3: mESC (Mouse embryonic stem cell) whole cell lysate at 20 µg
Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 5: A-375 (Human malignant melanoma cell line) whole cell lysate at 20 µg
Lane 6: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa
![Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--western-blot-img145614.jpg "Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
Western blot - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)
This data was developed using Anti-RNF40 antibody [EPR18511] ab191309, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the MW shown by commercial antibodies from other suppliers.
All lanes: Western blot - Anti-RNF40 antibody [EPR18511] (Anti-RNF40 antibody [EPR18511] ab191309) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 130 kDa
Exposure time: 15s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25259.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)
This data was developed using Anti-RNF40 antibody [EPR18511] ab191309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RNF40 with Anti-RNF40 antibody [EPR18511] ab191309 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on epithelial cells of human colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunoprecipitation-img53063.jpg "Immunoprecipitation - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
Immunoprecipitation - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)
This data was developed using Anti-RNF40 antibody [EPR18511] ab191309, the same antibody clone in a different buffer formulation.RNF40 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with Anti-RNF40 antibody [EPR18511] ab191309 at 1/80 dilution. Western blot was performed from the immunoprecipitate using Anti-RNF40 antibody [EPR18511] ab191309 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution. Lane 1: HeLa whole cell lysate 10ug (Input). Lane 2: Anti-RNF40 antibody [EPR18511] ab191309 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RNF40 antibody [EPR18511] ab191309 in HeLa whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.All lanes: Immunoprecipitation - Anti-RNF40 antibody [EPR18511] (Anti-RNF40 antibody [EPR18511] ab191309)
Predicted band size: 113 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70142.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)
This data was developed using Anti-RNF40 antibody [EPR18511] ab191309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling RNF40 with Anti-RNF40 antibody [EPR18511] ab191309 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on epithelial cells of mouse colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129585.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)
This data was developed using Anti-RNF40 antibody [EPR18511] ab191309, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling RNF40 with Anti-RNF40 antibody [EPR18511] ab191309 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on epithelial cells of rat colon is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70142.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058), expandable thumbnail](https://content.abcam.com/products/images/rnf40-antibody-epr18511-bsa-and-azide-free-ab251058--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129585.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF40 antibody [EPR18511] - BSA and Azide free (ab251058)")
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