Rabbit Recombinant Monoclonal RNF5/NG2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IP and reacts with Human samples.
Images
![Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--western-blot-img408774.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067)")
![Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--western-blot-img408773.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067)")
![Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--western-blot-img408775.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067)")
![Flow Cytometry (Intracellular) - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--flow-cytometry-intracellular-img408771.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067)")
![Immunoprecipitation - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--immunoprecipitation-img408772.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (AB308067)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt (Intra) | IP | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The observed MW of WB-3 is 45 kDa because the sample is recombinant protein. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes The observed MW of WB-3 is 45 kDa because the sample is recombinant protein. |
Species Rat | Dilution info - | Notes The observed MW of WB-3 is 45 kDa because the sample is recombinant protein. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Membrane-bound E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins (PubMed:11329381, PubMed:12861019, PubMed:16176924, PubMed:19269966, PubMed:19285439). May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4 (PubMed:11329381). Mediates ubiquitination of PXN/paxillin,thereby regulating cell motility and localization of PXN/paxillin (PubMed:12861019). Catalyzes ubiquitination of Salmonella type III secreted protein sopA (PubMed:16176924). Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N (PubMed:19269966). Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses (PubMed:19285439). Catalyzes ubiquitination and subsequent degradation of ATG4B, thereby inhibiting autophagy (PubMed:23093945).
Alternative names
G16, NG2, RMA1, RNF5, E3 ubiquitin-protein ligase RNF5, RING finger protein 5, Ram1 homolog, HsRma1
Recommended products
Rabbit Recombinant Monoclonal RNF5/NG2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IP and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27133-30
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--western-blot-img408774.jpg "Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)")
Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)
This data was developed using 308066, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] (Anti-RNF5/NG2 antibody [EPR27133-30] ab308066) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: Human colon tissue lysate at 20 µg
Lane 3: Human colon cancer tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 19 kDa
Exposure time: 70s
![Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--western-blot-img408773.jpg "Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)")
Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)
This data was developed using 308066, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] (Anti-RNF5/NG2 antibody [EPR27133-30] ab308066) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: 293T transfected with siRNA specifically targeting RNF5/NG2 whole cell lysate at 20 µg
Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 19 kDa
Exposure time: 180s
![Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--western-blot-img408775.jpg "Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)")
Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)
This data was developed using 308066, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with human RNF185.
In Western blot, anti-GST antibody (Anti-GST antibody [EPR4236] ab111947) staining at 1/5000 dilution.
Exposure time: 180 secondsAll lanes: Western blot - Anti-RNF5/NG2 antibody [EPR27133-30] (Anti-RNF5/NG2 antibody [EPR27133-30] ab308066) at 1/1000 dilution
Lane 1: Human RNF5 recombinant protein
Lane 2: Human RNF185 recombinant protein
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 45 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--flow-cytometry-intracellular-img408771.jpg "Flow Cytometry (Intracellular) - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)")
Flow Cytometry (Intracellular) - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)
This data was developed using Anti-RNF5/NG2 antibody [EPR27133-30] ab308066, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling RNF5/NG2 with Anti-RNF5/NG2 antibody [EPR27133-30] ab308066 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or Anti-RNF5/NG2 antibody [EPR27133-30] ab308066.![Immunoprecipitation - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067), expandable thumbnail](https://content.abcam.com/products/images/rnf5-ng2-antibody-epr27133-30-bsa-and-azide-free-ab308067--immunoprecipitation-img408772.jpg "Immunoprecipitation - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)")
Immunoprecipitation - Anti-RNF5/NG2 antibody [EPR27133-30] - BSA and Azide free (ab308067)
This data was developed using Anti-RNF5/NG2 antibody [EPR27133-30] ab308066, the same antibody clone in a different buffer formulation.
RNF5/NG2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug with Anti-RNF5/NG2 antibody [EPR27133-30] ab308066 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-RNF5/NG2 antibody [EPR27133-30] ab308066 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: Anti-RNF5/NG2 antibody [EPR27133-30] ab308066 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-RNF5/NG2 antibody [EPR27133-30] ab308066 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.All lanes: Immunoprecipitation - Anti-RNF5/NG2 antibody [EPR27133-30] (Anti-RNF5/NG2 antibody [EPR27133-30] ab308066) at 1/30 dilution
All lanes: HeLa whole cell lysate at 10 µg
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 19 kDa
Exposure time: 10s
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