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AB204506

Anti-RNF6 antibody

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(6 Publications)

Rabbit Polyclonal RNF6 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RNF6 aa 300 to C-terminus.

View Alternative Names

SPG2, RNF6, E3 ubiquitin-protein ligase RNF6

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF6 antibody (AB204506)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF6 antibody (AB204506)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue labeling RNF6 with ab204506 at 1/20 dilution.

Western blot - Anti-RNF6 antibody (AB204506)
  • WB

Supplier Data

Western blot - Anti-RNF6 antibody (AB204506)

All lanes:

Western blot - Anti-RNF6 antibody (ab204506) at 1/100 dilution

Lane 1:

Negative Control

Lane 2:

RNF6 over-expression HEK293T cell lysate

Predicted band size: 78 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RNF6 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y252

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover (By similarity). Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity (PubMed : 19345326). May also bind DNA and function as a transcriptional regulator (By similarity). Mediates polyubiquitination of QKI in macrophages, leading to its degradation (By similarity).
See full target information RNF6
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35681428

2022

Therapeutic Validation of GEF-H1 Using a De Novo Designed Inhibitor in Models of Retinal Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Clare Mills,Sandra A Hemkemeyer,Zerin Alimajstorovic,Chantelle Bowers,Malihe Eskandarpour,John Greenwood,Virginia Calder,A W Edith Chan,Paul J Gane,David L Selwood,Karl Matter,Maria S Balda

Pathology oncology research : POR 28:1610273 PubMed35369571

2022

RING-Finger Protein 6 promotes Drug Resistance in Retinoblastoma JAK2/STAT3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Chai,Shoufeng Jiao,Xin Peng,Qiang Gan,Leifeng Chen,Xiaolu Hu,Liang Hao,Shouhua Zhang,Qiang Tao

Diabetes, metabolic syndrome and obesity : targets and therapy 15:93-102 PubMed35046680

2022

RNF6 Targeted by miR-26a-5p Protects Pancreatic β-Cell Function Against Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Shengxun Zhao,Xuyan Zhang,Sheng Ding,Yancheng Xu

Experimental and therapeutic medicine 23:118 PubMed34970341

2022

RNF6 promotes the migration and invasion of breast cancer by promoting the ubiquitination and degradation of MST1.

Applications

Unspecified application

Species

Unspecified reactive species

Yajuan Huang,Yufeng Zou,Zhigang Jie

FEBS open bio 11:2041-2049 PubMed34081837

2021

Knockdown of RNF6 inhibits HeLa cervical cancer cell growth via suppression of MAPK/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Zhu,He Bai,Mingzhu Mu,Yuanyuan Xue,Zhao Duan

Aging 12:25564-25580 PubMed33264103

2020

TRIM27 acts as an oncogene and regulates cell proliferation and metastasis in non-small cell lung cancer through SIX3-β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyuan Liu,Ying Tian,Ying Zheng,Yao Cheng,Danjie Zhang,Jiantao Jiang,Shaomin Li
View all publications
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Product promise

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