Rabbit Polyclonal ROBO1 antibody. Suitable for ELISA, WB, IHC-P, IHC-Fr and reacts with Synthetic peptide, Mouse, Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human ROBO1 aa 1600-1650 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- International journal of molecular sciences 24:2023Local Administration of Minocycline Improves Nerve Regeneration in Two Rat Nerve Injury Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOwein Guillemot-Legris,Gedion Girmahun,Rebecca J Shipley,James B PhillipsPubMed 37569473
- Journal of biochemical and molecular toxicology 37:e234362023Matrine suppresses liver cancer progression and the Warburg effect by regulating the circROBO1/miR-130a-5p/ROBO1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengchun Huang,Hongwei Li,Qihua Li,Xuehong Chen,Ruizhen Liu,Xinfeng ChangPubMed 37376914
- Parasites & vectors 16:1842023MicroRNA-29a-3p prevents Schistosoma japonicum-induced liver fibrosis by targeting Roundabout homolog 1 in hepatic stellate cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyan Kong,Qiqin Song,Wenjiang Hu,Shusen Guo,Dandan Xiang,Shuaiwen Huang,Xin Xu,Jinan He,Lanyue Pan,Ran Tao,Haijing Yu,Jiaquan HuangPubMed 37280619
- FEBS letters 597:1290-12992023A Pellino-2 variant is associated with constitutive NLRP3 inflammasome activation in a family with ocular pterygium-digital keloid dysplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIleana Cristea,Hugo Abarca,Anne E Christensen Mellgren,Milana Trubnykova,Roya Mehrasa,Dorien J M Peters,Gunnar Houge,Raoul C M Hennekam,Eyvind Rødahl,Ove Bruland,Cecilie BredrupPubMed 36776133
- Frontiers in pharmacology 13:8517462022Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Feng Feng,Jian-Feng Lei,Man-Zhong Li,Yu Zhan,Le Yang,Yun Lu,Ming-Cong Li,Yu-Ming Zhuang,Lei Wang,Hui ZhaoPubMed 35559236
- Cellular & molecular biology letters 27:222022miR-152-3p impedes the malignant phenotypes of hepatocellular carcinoma by repressing roundabout guidance receptor 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Yin,Haonan ZhaoPubMed 35236289
- Gland surgery 10:2956-29652021ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang Hoon Kim,Tae-Jung Kim,Dongho Shin,Kyung Jae Hur,Sung-Hoo Hong,Ji Youl Lee,U-Syn HaPubMed 34804883
- Blood cancer discovery 2:338-3532021ROBO1 Promotes Homing, Dissemination, and Survival of Multiple Myeloma within the Bone Marrow Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiada Bianchi,Peter G Czarnecki,Matthew Ho,Aldo M Roccaro,Antonio Sacco,Yawara Kawano,Annamaria Gullà,Anil Aktas Samur,Tianzeng Chen,Kenneth Wen,Yu-Tzu Tai,Maria Moscvin,Xinchen Wu,Gulden Camci-Unal,Matteo C Da Vià,Niccolo' Bolli,Tomasz Sewastianik,Ruben D Carrasco,Irene M Ghobrial,Kenneth C AndersonPubMed 34268498
- Carcinogenesis 42:1046-10552021Circular RNA_0001495 increases Robo1 expression by sponging microRNA-527 to promote the proliferation, migration and invasion of bladder cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiuwei Feng,Dongmei Fu,Lei Gao,Hepeng Cheng,Chaoyang Zhu,Guangwei ZhangPubMed 34021307
- Experimental and therapeutic medicine 21:3982021Long non-coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR-146b-5p/Robo1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Jian,Qi FanPubMed 33680120
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ROBO1 aa 1600-1650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6N7
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Reactivity data
Select an application| ELISA | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected | Tested |
| Synthetic peptide | Tested | Not recommended | Not recommended | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/30000 - 1/60000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2-10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
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Target data
Function
Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development (By similarity).
Alternative names
DUTT1, ROBO1, Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1
Recommended products
Rabbit Polyclonal ROBO1 antibody. Suitable for ELISA, WB, IHC-P, IHC-Fr and reacts with Synthetic peptide, Mouse, Human samples. Cited in 54 publications. Immunogen corresponding to Synthetic Peptide within Human ROBO1 aa 1600-1650 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ROBO1 aa 1600-1650 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y6N7
- Purification technique
- Affinity purification Immunogen
- Specificity
Some batches may cross-react with Robo2 and Robo3. Please see abreview for more details.
- Concentration
- Purification notes
This affinity purified antibody is directed against human ROBO-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Roundabout homolog 1 (Robo1) also known as DUTT1 is a transmembrane receptor protein weighing approximately 181 kDa. It is part of the Robo receptor family specifically involved in axon guidance and neuronal migration. Robo1 primarily appears in neuronal tissues and other tissues like the lungs kidneys and prostate. Its role involves dynamic interactions with several ligands such as Slit proteins leading to the modulation of cellular pathways.
- Biological function summary
Robo1 interacts with Slit proteins to control processes like repulsion of axonal growth cones during neuronal development. The protein acts as an important player in guiding neuronal connections and establishing boundaries. Robo1 does not function alone forming complexes with other receptors and cell adhesion molecules to execute its biological roles effectively. Structurally this receptor facilitates cellular responses to environmental cues through its cytoplasmic signaling cascade.
- Pathways
Robo1 engages in the Slit-Robo signaling pathway essential for axonal guidance and cell migration. This pathway involves several key interactions with proteins such as DCC (Deleted in Colorectal Carcinoma) and Netrin impacting neuronal positioning and differentiation. Additionally Robo1 influences cellular orientation and tissue architecture by participating in the actin cytoskeleton remodeling pathway further demonstrating its significance in neuronal patterning.
- Associated diseases and disorders
Robo1 has links to cancer and neurodevelopmental disorders like autism spectrum disorders. The dysregulation of Robo1 expression has associations with aggressive tumor growth particularly in breast and lung cancers where altered signaling influences metastatic potential. Furthermore Robo1 interacts with proteins like Slit2 in cancer progression highlighting its importance in these conditions. In neurodevelopmental disorders altered Robo1 signaling can affect neural circuit formation contributing to changes seen in conditions like autism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Robo1 antibody (ab7279)
All lanes: Western blot - Anti-Robo1 antibody (ab7279) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 181 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo1 antibody (ab7279)
This image shows staining of human brain tissue.
Immunohistochemistry (Frozen sections) - Anti-Robo1 antibody (ab7279)
Immunohistochemistry analysis of mouse lung tissue sections (adult, frozen 100 μm wholemount sections) labelling Robo1 with ab7279 at 1/50 dilution. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.
This image is courtesy of an anonymous customer review.Western blot - Anti-Robo1 antibody (ab7279)
All lanes: Western blot - Anti-Robo1 antibody (ab7279) at 1/1000 dilution
All lanes: HEK 293T cells at 25 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 181 kDa
Western blot - Anti-Robo1 antibody (ab7279)
Primary antibody incubated overnight at 4C.
Blocking Buffer: 1% Casein-TTBS for 30 min at room temperature.
All lanes: Western blot - Anti-Robo1 antibody (ab7279) at 1 µg/mL
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg
Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Lane 5: A431 (human epidermoid carcinoma cell line) whole cell lysate at 35 µg
Lane 6: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg
Lane 7: LNCaP (human prostate cancer cell line) whole cell lysate at 35 µg
Lane 8: MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 35 µg
Lane 9: Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 35 µg
Lane 10: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 35 µg
Lane 11: A-172 (human brain glioblastoma) whole cell lysate at 35 µg
Lane 12: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 35 µg
SecondaryAll lanes: Peroxidase Rabbit secondary antibody for 60 minutes at room temperature at 1/30000 dilution
Predicted band size: 181 kDa
Observed band size: 181 kDa
ELISA - Anti-Robo1 antibody (ab7279)
ELISA results of purified ab7279 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated secondary and TMB ELISA Peroxidase Substrate.
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