Anti-Robo1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo1 antibody (AB7279)

This image shows staining of human brain tissue.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Robo1 antibody (AB7279)

Immunohistochemistry analysis of mouse lung tissue sections (adult, frozen 100 μm wholemount sections) labelling Robo1 with ab7279 at 1/50 dilution. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.

• ELISA

Supplier Data

ELISA - Anti-Robo1 antibody (AB7279)

ELISA results of purified ab7279 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated secondary and TMB ELISA Peroxidase Substrate.

• WB

AbReview34149****

Western blot - Anti-Robo1 antibody (AB7279)

All lanes:

Western blot - Anti-Robo1 antibody (ab7279) at 1/1000 dilution

All lanes:

HEK 293T cells at 25 µg

Predicted band size: 181 kDa

true

This image is courtesy of an anonymous Abreview.

• WB

Supplier Data

Western blot - Anti-Robo1 antibody (AB7279)

Primary antibody incubated overnight at 4C.

Blocking Buffer : 1% Casein-TTBS for 30 min at room temperature.

All lanes:

Western blot - Anti-Robo1 antibody (ab7279) at 1 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

Lane 5:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 35 µg

Lane 6:

A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 7:

LNCaP (human prostate cancer cell line) whole cell lysate at 35 µg

Lane 8:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 35 µg

Lane 9:

Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 35 µg

Lane 10:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 35 µg

Lane 11:

A-172 (human brain glioblastoma) whole cell lysate at 35 µg

Lane 12:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 35 µg

Secondary

All lanes:

Peroxidase Rabbit secondary antibody for 60 minutes at room temperature at 1/30000 dilution

Predicted band size: 181 kDa

Observed band size: 181 kDa

false

• WB

Supplier Data

Western blot - Anti-Robo1 antibody (AB7279)

All lanes:

Western blot - Anti-Robo1 antibody (ab7279) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 181 kDa

false