AB85312

Anti-Robo1 antibody

(3 Reviews)

(6 Publications)

Rabbit Polyclonal ROBO1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

DUTT1, ROBO1, Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1

1 Images

Unknown

Western blot - Anti-Robo1 antibody (AB85312)

Human Roundabout homolog 1 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Robo1 antibody (ab85312) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 181 kDa

Observed band size: 190 kDa

true

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Roundabout homolog 1 (Robo1) also known as DUTT1 is a transmembrane receptor protein weighing approximately 181 kDa. It is part of the Robo receptor family specifically involved in axon guidance and neuronal migration. Robo1 primarily appears in neuronal tissues and other tissues like the lungs kidneys and prostate. Its role involves dynamic interactions with several ligands such as Slit proteins leading to the modulation of cellular pathways.

Biological function summary

Robo1 interacts with Slit proteins to control processes like repulsion of axonal growth cones during neuronal development. The protein acts as an important player in guiding neuronal connections and establishing boundaries. Robo1 does not function alone forming complexes with other receptors and cell adhesion molecules to execute its biological roles effectively. Structurally this receptor facilitates cellular responses to environmental cues through its cytoplasmic signaling cascade.

Pathways

Robo1 engages in the Slit-Robo signaling pathway essential for axonal guidance and cell migration. This pathway involves several key interactions with proteins such as DCC (Deleted in Colorectal Carcinoma) and Netrin impacting neuronal positioning and differentiation. Additionally Robo1 influences cellular orientation and tissue architecture by participating in the actin cytoskeleton remodeling pathway further demonstrating its significance in neuronal patterning.

Robo1 has links to cancer and neurodevelopmental disorders like autism spectrum disorders. The dysregulation of Robo1 expression has associations with aggressive tumor growth particularly in breast and lung cancers where altered signaling influences metastatic potential. Furthermore Robo1 interacts with proteins like Slit2 in cancer progression highlighting its importance in these conditions. In neurodevelopmental disorders altered Robo1 signaling can affect neural circuit formation contributing to changes seen in conditions like autism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed : 10102268, PubMed : 24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed : 24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed : 26529257). May be required for lung development (By similarity).

See full target information ROBO1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 13:701 PubMed31354412

2019

An MRI Study of Neurovascular Restorative After Combination Treatment With Enteric-Coated Capsule and Enriched Environment in Rats After Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhan,Man-Zhong Li,Le Yang,Xue-Feng Feng,Qiu-Xia Zhang,Nan Zhang,Yuan-Yuan Zhao,Hui Zhao

Breast cancer research : BCR 20:86 PubMed30075800

2018

Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Smeda,Anna Kieronska,Mateusz G Adamski,Bartosz Proniewski,Magdalena Sternak,Tasnim Mohaissen,Kamil Przyborowski,Katarzyna Derszniak,Dawid Kaczor,Marta Stojak,Elzbieta Buczek,Agnieszka Jasztal,Joanna Wietrzyk,Stefan Chlopicki

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 43:900-911 PubMed29154364

2017

Non-Contingent Exposure to Amphetamine in Adolescence Recruits miR-218 to Regulate Dcc Expression in the VTA.

Applications

Unspecified application

Species

Unspecified reactive species

Santiago Cuesta,José Maria Restrepo-Lozano,Steven Silvestrin,Dominique Nouel,Angélica Torres-Berrío,Lauren M Reynolds,Andreas Arvanitogiannis,Cecilia Flores

Journal of cell science 130:2459-2467 PubMed28596239

2017

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ja Huang,Paula Schiapparelli,Kristen Kozielski,Jordan Green,Emily Lavell,Hugo Guerrero-Cazares,Alfredo Quinones-Hinojosa,Peter Searson

Scientific reports 5:16833 PubMed26582347

2015

Slit2N Inhibits Transmission of HIV-1 from Dendritic Cells to T-cells by Modulating Novel Cytoskeletal Elements.

Applications

Unspecified application

Species

Unspecified reactive species

Ashutosh Shrivastava,Anil Prasad,Paula M Kuzontkoski,Jinlong Yu,Jerome E Groopman

Nature neuroscience 18:36-45 PubMed25485759

2014

PlexinA1 is a new Slit receptor and mediates axon guidance function of Slit C-terminal fragments.

Applications

Unspecified application

Species

Unspecified reactive species

Céline Delloye-Bourgeois,Arnaud Jacquier,Camille Charoy,Florie Reynaud,Homaira Nawabi,Karine Thoinet,Karine Kindbeiter,Yutaka Yoshida,Yvrick Zagar,Youxin Kong,Yvonne E Jones,Julien Falk,Alain Chédotal,Valérie Castellani

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Robo1 antibody

Western blot - Anti-Robo1 antibody (AB85312)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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