Rabbit Polyclonal ROBO1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications.
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- Frontiers in neuroscience 13:7012019An MRI Study of Neurovascular Restorative After Combination Treatment With Enteric-Coated Capsule and Enriched Environment in Rats After Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhan et. al.PubMed 31354412
- Breast cancer research : BCR 20:862018Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Smeda et. al.PubMed 30075800
- Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 43:900-9112017Non-Contingent Exposure to Amphetamine in Adolescence Recruits miR-218 to Regulate Dcc Expression in the VTA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSantiago Cuesta,José Maria Restrepo-Lozano,Steven Silvestrin,Dominique Nouel,Angélica Torres-Berrío,Lauren M Reynolds,Andreas Arvanitogiannis,Cecilia FloresPubMed 29154364
- Journal of cell science 130:2459-24672017Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Ja Huang et. al.PubMed 28596239
- Scientific reports 5:168332015Slit2N Inhibits Transmission of HIV-1 from Dendritic Cells to T-cells by Modulating Novel Cytoskeletal Elements.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshutosh Shrivastava et. al.PubMed 26582347
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
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Target data
Function
Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development (By similarity).
Alternative names
DUTT1, ROBO1, Roundabout homolog 1, Deleted in U twenty twenty, H-Robo-1
Recommended products
Rabbit Polyclonal ROBO1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Roundabout homolog 1 (Robo1) also known as DUTT1 is a transmembrane receptor protein weighing approximately 181 kDa. It is part of the Robo receptor family specifically involved in axon guidance and neuronal migration. Robo1 primarily appears in neuronal tissues and other tissues like the lungs kidneys and prostate. Its role involves dynamic interactions with several ligands such as Slit proteins leading to the modulation of cellular pathways.
- Biological function summary
Robo1 interacts with Slit proteins to control processes like repulsion of axonal growth cones during neuronal development. The protein acts as an important player in guiding neuronal connections and establishing boundaries. Robo1 does not function alone forming complexes with other receptors and cell adhesion molecules to execute its biological roles effectively. Structurally this receptor facilitates cellular responses to environmental cues through its cytoplasmic signaling cascade.
- Pathways
Robo1 engages in the Slit-Robo signaling pathway essential for axonal guidance and cell migration. This pathway involves several key interactions with proteins such as DCC (Deleted in Colorectal Carcinoma) and Netrin impacting neuronal positioning and differentiation. Additionally Robo1 influences cellular orientation and tissue architecture by participating in the actin cytoskeleton remodeling pathway further demonstrating its significance in neuronal patterning.
- Associated diseases and disorders
Robo1 has links to cancer and neurodevelopmental disorders like autism spectrum disorders. The dysregulation of Robo1 expression has associations with aggressive tumor growth particularly in breast and lung cancers where altered signaling influences metastatic potential. Furthermore Robo1 interacts with proteins like Slit2 in cancer progression highlighting its importance in these conditions. In neurodevelopmental disorders altered Robo1 signaling can affect neural circuit formation contributing to changes seen in conditions like autism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Robo1 antibody (ab85312)
Human Roundabout homolog 1 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-Robo1 antibody (ab85312) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Brain (Mouse) Tissue Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 181 kDa
Observed band size: 190 kDa
Exposure time: 20min
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