AB244331

Anti-Robo2 antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal ROBO2 antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ROBO2 aa 900-1100.

View Alternative Names

KIAA1568, ROBO2, Roundabout homolog 2

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-Robo2 antibody (AB244331)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Robo2 antibody (AB244331)

Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Robo2 (green) in the nucleus with ab244331 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

Immunohistochemical analysis of human cerebral cortex tissue labeling Robo2 in neuronal processes in neuropil with ab244331 at 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

Immunohistochemical analysis of human placenta tissue labeling Robo2 in the cytoplasm of trophoblastic cels with ab244331 at 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

Immunohistochemical analysis of human kidney tissue labeling Robo2 with ab244331 at 1/20 dilution. Negative control.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

Immunohistochemical analysis of mouse embryo E14 tissue labeling Robo2 in developing olfatory bulb and olfactory nerve with ab244331 at 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo2 antibody (AB244331)

Immunohistochemical analysis of human liver tissue labeling Robo2 (very weak positivity) with ab244331 at 1/20 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF

Immunogen

Recombinant Fragment Protein within Human ROBO2 aa 900-1100. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Robo2 also known as Roundabout Homolog 2 is a protein with significant roles in axon guidance. This protein has a molecular mass of approximately 147 kDa and is a member of the Robo receptor family. It is primarily expressed in the nervous system including areas such as the spinal cord brain and olfactory bulb. Robo2 interacts with Slit proteins to mediate the repulsive guidance of axons which is fundamental for the formation of connections in the developing nervous system.

Biological function summary

Robo2 functions in the nervous system to guide axons and establish neuron networks. It plays a role in cellular processes like migration and axonal pathfinding ensuring proper connectivity. Robo2 operates as a receptor and does not form part of a larger complex by itself but it does have interactions with other molecules. By binding with Slit proteins Robo2 helps repel axons away from certain areas aiding in the clear mapping of axonal trajectories.

Pathways

Robo2 participates in axon guidance pathways including the Slit-Robo signaling pathway. This pathway is fundamental in nervous system development and is responsible for steering axonal growth direction. Within these pathways Robo2 displays interaction with proteins like neuron-specific Slit proteins which are secreted molecules helping to establish gradients that direct axon shrinkage or extension.

Robo2 is linked to conditions like congenital heart defects and urofacial syndrome. Abnormal expression or mutations in the Robo2 gene may disrupt normal axonal pathfinding leading to developmental issues. Robo2 has also shown associations with other proteins like Slit2 which together contribute to defective axon guidance when altered underlying certain neurodevelopmental disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.

See full target information ROBO2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pharmacogenomics and personalized medicine 16:1041-1053 PubMed38058295

2023

Identification of P3H1 as a Predictive Prognostic Biomarker for Bladder Urothelial Carcinoma Based on the Cancer Genome Atlas Database.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfeng Zhang,Yang Chen,Zhiming Chen,Xinye Zhou,Shaochuan Chen,Kaijian Lan,Zhiping Wang,Yonghai Zhang

View all publications

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Product promise

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