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AB180824

Anti-Robo4 antibody

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(2 Publications)

Guinea pig Polyclonal ROBO4 antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Transfected cell lysate - Mouse samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ROBO4.

View Alternative Names

UNQ421/PRO3674, ROBO4, Roundabout homolog 4, Magic roundabout

1 Images
Western blot - Anti-Robo4 antibody (AB180824)
  • WB

Supplier Data

Western blot - Anti-Robo4 antibody (AB180824)

All lanes:

Western blot - Anti-Robo4 antibody (ab180824)

Lane 1:

HEK 293 cells transiently transfected with mouse Robo4 (reduced conditions) at 3 µg

Lane 2:

HEK 293 cell lysate (untransfected) at 3 µg

Lane 3:

HEK 293 cell lysate (untransfected) (non-reduced) at 3 µg

Lane 4:

HEK 293 cells transiently transfected with mouse Robo4 (non-reduced) at 3 µg

Lane 5:

HEK 293 cells transiently transfected with human Robo4 (reduced conditions) at 3 µg

Predicted band size: 107 kDa

false

Key facts

Host species

Guinea pig

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Native Full Length Protein corresponding to Human ROBO4.

Q8WZ75

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Reactivity data

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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Roundabout homolog 4 (Robo4) is a protein weighing approximately 107 kDa. It belongs to the immunoglobulin superfamily and is sometimes referred to as Magic Roundabout. Robo4 primarily gets expressed in endothelial cells playing an important function in vascular biology. It is characterized by having two immunoglobulin-like domains and two fibronectin type III domains making it distinct among the other Robo family members.
Biological function summary

Robo4 stabilizes the vascular network interacting actively with the guidance molecule Slit proteins. It is not part of a larger protein complex but engages directly with these ligands to influence endothelial cell migration and vascular permeability. Its role is significant in maintaining the structural integrity of blood vessels ensuring the appropriate cellular responses important for proper vascular function.

Pathways

Robo4 engages in the Slit-Robo signaling pathway that is essential for vascular development and remodeling. This pathway helps inhibit excessive endothelial migration and angiogenesis. Robo4 interacts with proteins like Slit2 which binds to it as a ligand to mediate these effects. Through this pathway Robo4 performs an important function in processes such as vascular homeostasis and angiogenesis regulation.

Robo4 appears to have connections to vascular-related conditions including diabetic retinopathy and age-related macular degeneration. The protein influences pathological angiogenesis which is a hallmark in the progression of these diseases. In these contexts Robo4's interaction with Slit2 proves important as it can restrain abnormal vascular growth offering potential therapeutic angles for treatment approaches focused on targeting the Slit-Robo pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity). Involved in the maintenance of endothelial barrier organization and function (PubMed : 30455415).
See full target information ROBO4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 23: PubMed33495827

2021

miR-140-5p regulates vascular smooth muscle cell viability, migration and apoptosis by targeting ROBO4 gene expression in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Luo,Yangmin Li,Hong Peng,Yu Zhao

Experimental cell research 368:13-23 PubMed29649427

2018

VEGFR-3 signaling is regulated by a G-protein activator, activator of G-protein signaling 8, in lymphatic endothelial cells.

Applications

Unspecified application

Species

Human

Miho Sakima,Hisaki Hayashi,Abdullah Al Mamun,Motohiko Sato
View all publications
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