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AB273519

Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free

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Rabbit Recombinant Monoclonal ROBO4 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples.

View Alternative Names

UNQ421/PRO3674, ROBO4, Roundabout homolog 4, Magic roundabout

1 Images
Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (AB273519)
  • Flow Cyt

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Flow Cytometry - Anti-Robo4 antibody [EPR23426-145] - BSA and Azide free (AB273519)

Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) (Left panel) / HUVEC (Human umbilical vein endothelial cell) (Right panel) cells labelling Robo4 with ab272734 at 1/50 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

Negative control : HeLa (PMID : 11076864).

Gated on viable cells.

Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272734).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23426-145

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab273519 is the carrier-free version of ab272734.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Roundabout homolog 4 (Robo4) is a protein weighing approximately 107 kDa. It belongs to the immunoglobulin superfamily and is sometimes referred to as Magic Roundabout. Robo4 primarily gets expressed in endothelial cells playing an important function in vascular biology. It is characterized by having two immunoglobulin-like domains and two fibronectin type III domains making it distinct among the other Robo family members.
Biological function summary

Robo4 stabilizes the vascular network interacting actively with the guidance molecule Slit proteins. It is not part of a larger protein complex but engages directly with these ligands to influence endothelial cell migration and vascular permeability. Its role is significant in maintaining the structural integrity of blood vessels ensuring the appropriate cellular responses important for proper vascular function.

Pathways

Robo4 engages in the Slit-Robo signaling pathway that is essential for vascular development and remodeling. This pathway helps inhibit excessive endothelial migration and angiogenesis. Robo4 interacts with proteins like Slit2 which binds to it as a ligand to mediate these effects. Through this pathway Robo4 performs an important function in processes such as vascular homeostasis and angiogenesis regulation.

Robo4 appears to have connections to vascular-related conditions including diabetic retinopathy and age-related macular degeneration. The protein influences pathological angiogenesis which is a hallmark in the progression of these diseases. In these contexts Robo4's interaction with Slit2 proves important as it can restrain abnormal vascular growth offering potential therapeutic angles for treatment approaches focused on targeting the Slit-Robo pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins (By similarity). Involved in the maintenance of endothelial barrier organization and function (PubMed : 30455415).
See full target information ROBO4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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