Anti-ROCK1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody (AB97592)

Immunofluorescence analysis of ICC-IF HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling ROCK1 protein at nucleus and Golgi apparatus using ab97592 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker was stained at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

• IP

Supplier Data

Immunoprecipitation - Anti-ROCK1 antibody (AB97592)

Samples were separated by 5% SDS-PAGE. The immunoprecipitated ROCK1 protein was detected by ab97592 diluted at 1/ 500 dilution. Anti-rabbit IgG (HRP) was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-ROCK1 antibody (ab97592) at 1/500 dilution

Lane 1:

Control with 3 μg of pre-immune rabbit IgG

Lane 2:

A431 whole cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 158 kDa

false

• WB

Supplier Data

Western blot - Anti-ROCK1 antibody (AB97592)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ROCK1 antibody (ab97592) at 1/1000 dilution

Lane 1:

Non-transfected HeLa whole cell extracts at 30 µg

Lanes 2 - 3:

ROCK1 shRNA ransfected HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 158 kDa

false

• WB

Supplier Data

Western blot - Anti-ROCK1 antibody (AB97592)

5% SDS-PAGE

All lanes:

Western blot - Anti-ROCK1 antibody (ab97592) at 1/1000 dilution

Lane 1:

Wild-type HeLa whole cell lysate at 30 µg

Lane 2:

ROCK1 knockout HeLa whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 158 kDa

false

• WB

Supplier Data

Western blot - Anti-ROCK1 antibody (AB97592)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ROCK1 antibody (ab97592) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 158 kDa

false

• WB

Supplier Data

Western blot - Anti-ROCK1 antibody (AB97592)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-ROCK1 antibody (ab97592) at 1/1000 dilution

Lane 1:

NIH-3T3 whole cell extract at 30 µg

Lane 2:

C2Cl2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 158 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody (AB97592)

Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labelling ROCK1 with ab97592 antibody at 1/500 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody (AB97592)

Immunofluorescence staining of paraformaldehyde-fixed (4% paraformaldehyde, 15 minutes at RT) HeLa cells stained for ROCK1 with ab97592 at 1 : 500 dilution. Red : alpha Tubulin, a cytoskeleton marker, stained by an alpha Tubulin antibody diluted at 1 : 500.
Blue : Hoechst 33342 staining.

• WB

Supplier Data

Western blot - Anti-ROCK1 antibody (AB97592)

All lanes:

Western blot - Anti-ROCK1 antibody (ab97592) at 1/500 dilution

All lanes:

Rat brain lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 158 kDa

false