Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--western-blot-img61147.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img177607.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799)")
![Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--western-blot-img177439.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799)")
![Immunoprecipitation - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--immunoprecipitation-img3398.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799)")
![Flow Cytometry (Intracellular) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--flow-cytometry-intracellular-img170738.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (AB230799)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
3 products for Alternative Version
Target data
Function
Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed:10436159, PubMed:10652353, PubMed:11018042, PubMed:11283607, PubMed:17158456, PubMed:18573880, PubMed:19131646, PubMed:8617235, PubMed:9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed:10436159, PubMed:10652353, PubMed:11018042, PubMed:11283607, PubMed:17158456, PubMed:18573880, PubMed:19131646, PubMed:23093407, PubMed:23355470, PubMed:8617235, PubMed:9722579). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed:18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed:19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed:19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed:21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed:19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).
Alternative names
Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, p160 ROCK-1, ROCK-I, p160ROCK, ROCK1
Recommended products
Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR638Y
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab230799 is the carrier-free version of Anti-ROCK1 antibody [EPR638Y] ab134181.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--western-blot-img61147.jpg "Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)
This WB data was generated using the same anti-ROCK1 antibody clone, EPR638Y, in a different buffer formulation (cat# Anti-ROCK1 antibody [EPR638Y] ab134181).
Lane 1: Wild type HAP1 whole cell lysate (40 μg)
Lane 2: ROCK1 knockout HAP1 whole cell lysate (40 μg)
Lane 3: HEK293 whole cell lysate (20 μg)
Lane 4: HeLa whole cell lysate (20 μg)Lanes 1 - 4: Merged signal (red and green). Green - Anti-ROCK1 antibody [EPR638Y] ab134181 observed at 165 kDa. Red - loading control, ab18058, observed at 130 kDa.
Anti-ROCK1 antibody [EPR638Y] ab134181 was shown to specifically react with ROCK1 when ROCK1 knockout samples were used. Wild-type and ROCK1 knockout samples were subjected to SDS-PAGE. Anti-ROCK1 antibody [EPR638Y] ab134181 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ROCK1 antibody [EPR638Y] (Anti-ROCK1 antibody [EPR638Y] ab134181)
Predicted band size: 158 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img177607.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)
This data was developed using Anti-ROCK1 antibody [EPR638Y] ab134181, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling ROCK1 with purified Anti-ROCK1 antibody [EPR638Y] ab134181 at 1:1200 (1.28 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--western-blot-img177439.jpg "Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)
This data was developed using Anti-ROCK1 antibody [EPR638Y] ab134181, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ROCK1 antibody [EPR638Y] (Anti-ROCK1 antibody [EPR638Y] ab134181) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Mouse brain at 20 µg
Lane 3: Rat brain at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 158 kDa
Observed band size: 158 kDa
![Immunoprecipitation - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--immunoprecipitation-img3398.jpg "Immunoprecipitation - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
Immunoprecipitation - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)
Purified Anti-ROCK1 antibody [EPR638Y] ab134181 at 1/120 dilution (2μg) immunoprecipitating ROCK1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): Anti-ROCK1 antibody [EPR638Y] ab134181 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ROCK1 antibody [EPR638Y] ab134181 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 158 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ROCK1 antibody [EPR638Y] ab134181).All lanes: Immunoprecipitation - Anti-ROCK1 antibody [EPR638Y] (Anti-ROCK1 antibody [EPR638Y] ab134181)
Predicted band size: 158 kDa
![Flow Cytometry (Intracellular) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--flow-cytometry-intracellular-img170738.jpg "Flow Cytometry (Intracellular) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
Flow Cytometry (Intracellular) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)
This data was developed using Anti-ROCK1 antibody [EPR638Y] ab134181, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling ROCK1 with purified Anti-ROCK1 antibody [EPR638Y] ab134181 at 1/150 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img179749.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)
This data was developed using Anti-ROCK1 antibody [EPR638Y] ab134181, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling ROCK1 with purified Anti-ROCK1 antibody [EPR638Y] ab134181 at 1:1200 (1.28 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--western-blot-img7420.jpg "Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ROCK1 antibody [EPR638Y] ab134181).
Lanes 1- 2: Merged signal (red and green). Green - Anti-ROCK1 antibody [EPR638Y] ab134181 observed at 160 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.
Anti-ROCK1 antibody [EPR638Y] ab134181 was shown to react with ROCK1 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264780 (CRISPR/Cas9 edited cell lysate ab257642) lane below 160kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and ROCK1 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-ROCK1 antibody [EPR638Y] ab134181 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ROCK1 antibody [EPR638Y] (Anti-ROCK1 antibody [EPR638Y] ab134181) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ROCK1 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 158 kDa
Observed band size: 160 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img179749.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
![Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799), expandable thumbnail](https://content.abcam.com/products/images/rock1-antibody-epr638y-bsa-and-azide-free-ab230799--western-blot-img7420.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ROCK1 antibody [EPR638Y] - BSA and Azide free (ab230799)")
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