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Knockout Tested Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

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Images

Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (AB322252), expandable thumbnail
  • Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (AB322252), expandable thumbnail
  • Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (AB322252), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Alternative names

Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II, KIAA0619, ROCK2

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

Alternative names

Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II, KIAA0619, ROCK2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24575-35

Purification technique

Affinity purification Protein A

Specificity

This antibody does not cross-react with human ROCK1.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab322252 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify ROCK2. The recommended pair for sandwich ELISA is:

Capture: Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) ab322251, ROCK2 Capture Antibody (unconjugated)

Detector: ab322252, ROCK2 Detector Antibody (unconjugated)

The reference range value is 0.08-10 ng/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (ab322252), expandable thumbnail

    Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (ab322252)

    Sandwich ELISA of ROCK2 Antibody Pair - BSA and Azide free ab322250 with the capture antibody Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) ab322251 dilution at 2 ug/mL and detector antibody ab322252 dilution at 0.5 ug/mL.

  • Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (ab322252), expandable thumbnail

    Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (ab322252)

    Interpolated concentrations of native ROCK2 in human control wild type HAP1 (human near-haploid cell) and ROCK2 knockout HAP1 cell based on 500 ug/mL extract loads. The concentrations of ROCK2 were measured in duplicate and interpolated from the ROCK2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ROCK2 concentration was determined to be 4191.8 pg/mL in wild type HAP1 extract and undetectable in ROCK2 knockout HAP1 extract.

  • Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (ab322252), expandable thumbnail

    Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-35] - BSA and Azide free (Detector) (ab322252)

    Interpolated concentration of native ROCK2 was measured at different sample concentrations. Undiluted samples are as follows: HAP1 cell extract (500 ug/mL), NIH/3T3 cell extract (125 ug/mL) and C6 cell extract (125 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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