Skip to main content

Knockout Tested Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for Dot, WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Dot Blot - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (AB317247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (AB317247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (AB317247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (AB317247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (AB317247), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIHC-PFlow Cyt (Intra)IPICC/IF
Human
Expected
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Tested
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Alternative names

KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for Dot, WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR24575-76
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab317247 is the carrirer-free version of Anti-ROCK2 antibody [EPR24575-76] ab317246.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Dot Blot - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Dot Blot - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    ROCK2 Dot Blot staining using rabbit Anti-ROCK2 antibody

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    Dot blot analysis of ROCK2 using Anti-ROCK2 antibody [EPR24575-76] ab317246 at 1:1000 (0.494 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    This antibody does not cross-react with human ROCK1.

    All lanes: Dot Blot - Anti-ROCK2 antibody [EPR24575-76] (Anti-ROCK2 antibody [EPR24575-76] ab317246) at 1/1000 dilution

    Lane 1: Human ROCK1 recombinant fragment

    Lane 2: Human ROCK2 recombinant fragment

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 8s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded (A) Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell). (B) ROCK2 knockout HAP1. tissue labeling ROCK2 with Anti-ROCK2 antibody [EPR24575-76] ab317246 at 1/1000 (0.494 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) Wild-type HAP1, no staining on (B) ROCK2 knockout HAP1.

    The section was incubated with Anti-ROCK2 antibody [EPR24575-76] ab317246 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ROCK2 with Anti-ROCK2 antibody [EPR24575-76] ab317246 at 1/1000 (0.494 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat cerebrum.

    The section was incubated with Anti-ROCK2 antibody [EPR24575-76] ab317246 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse lung tumor tissue labeling ROCK2 with Anti-ROCK2 antibody [EPR24575-76] ab317246 at 1/1000 (0.494 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lung tumor.

    The section was incubated with Anti-ROCK2 antibody [EPR24575-76] ab317246 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ROCK2 with Anti-ROCK2 antibody [EPR24575-76] ab317246 at 1/1000 (0.494 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum.

    The section was incubated with Anti-ROCK2 antibody [EPR24575-76] ab317246 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ROCK2 with Anti-ROCK2 antibody [EPR24575-76] ab317246 at 1/1000 (0.494 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung carcinoma.

    The section was incubated with Anti-ROCK2 antibody [EPR24575-76] ab317246 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ROCK2 with Anti-ROCK2 antibody [EPR24575-76] ab317246 at 1/1000 (0.494 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum.

    The section was incubated with Anti-ROCK2 antibody [EPR24575-76] ab317246 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Western blot - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    In lanes 1-2, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation. In lanes 3-7, the lysate was stored at -80℃ prior to Western Blotting.

    Exposure time: Lanes 1-2: 37 seconds; Lanes 3-7: 6 seconds.

    All lanes: Western blot - Anti-ROCK2 antibody [EPR24575-76] (Anti-ROCK2 antibody [EPR24575-76] ab317246) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lanes 2 and 7: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 5: C2C12 (mouse myoblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 160 kDa

  • Western blot - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247), expandable thumbnail

    Western blot - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (ab317247)

    This data was developed using Anti-ROCK2 antibody [EPR24575-76] ab317246, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-ROCK2 antibody [EPR24575-76] ab317246 was shown to bind specifically to ROCK2. Target of interest was observed at 160 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in ROCK2 knockout cell line (lane 2).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ROCK2 antibody [EPR24575-76] (Anti-ROCK2 antibody [EPR24575-76] ab317246) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: ROCK2 knockout HAP1 whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 160 kDa, 36 kDa

    Exposure time: 70s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com