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AB322251

Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture)

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Knockout Tested Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

View Alternative Names

KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II

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Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) (AB322251)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) (AB322251)

Interpolated concentrations of native ROCK2 in human control wild type HAP1 (human near-haploid cell) and ROCK2 knockout HAP1 cell based on 500 ug/mL extract loads. The concentrations of ROCK2 were measured in duplicate and interpolated from the ROCK2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ROCK2 concentration was determined to be 4191.8 pg/mL in wild type HAP1 extract and undetectable in ROCK2 knockout HAP1 extract.

Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) (AB322251)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) (AB322251)

Sandwich ELISA of ab322250 with the capture antibody ab322251 dilution at 2 ug/mL and detector antibody ab322252 dilution at 0.5 ug/mL.

Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) (AB322251)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ROCK2 antibody [EPR24575-76] - BSA and Azide free (Capture) (AB322251)

Interpolated concentration of native ROCK2 was measured at different sample concentrations. Undiluted samples are as follows : HAP1 cell extract (500 ug/mL), NIH/3T3 cell extract (125 ug/mL) and C6 cell extract (125 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24575-76

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with human ROCK1.

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Reactivity data

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Product details

ab322251 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify ROCK2. The recommended pair for sandwich ELISA is:

Capture: ab322251, ROCK2 Capture Antibody (unconjugated)

Detector: ab322252, ROCK2 Detector Antibody (unconjugated)

The reference range value is 0.08-10 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
See full target information ROCK2
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Product promise

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