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Rabbit Recombinant Monoclonal ROCK2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 47 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.14% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Expected
Rat
Tested
Not recommended
Expected

Tested
Tested

Species

Mouse

Dilution info

1/10000 - 1/50000

Notes

-

Species

Rat

Dilution info

1/10000 - 1/50000

Notes

-

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Alternative names

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Rabbit Recombinant Monoclonal ROCK2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 47 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7141(B)

Purification technique

Affinity purification Protein A

Dissociation constant

8.7 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025), expandable thumbnail

    Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: ROCK2 knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: A10 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab125025 observed at 165 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab125025 was shown to specifically react with ROCK2 when ROCK2 knockout samples were used. Wild-type and ROCK2 knockout samples were subjected to SDS-PAGE. ab125025 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-ROCK2 antibody [EPR7141(B)] (AB125025)

    Predicted band size: 161 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] (ab125025), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling ROCK2 with purified ab125025 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) primary and ab150120 (AlexaFluor®594 goat anti-mouse) secondary both at 1/1000 dilution. Nuclei were counterstained with DAPI (blue).

    For negative control 1, rabbit primary antibody and ab150120 (anti-mouse) secondary antibody were used. For negative control 2, ab7291 (mouse primary antibody) was used followed by ab150077 (anti-rabbit secondary antibody).

  • Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025), expandable thumbnail

    Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)

    Lanes 1- 4: Merged signal (red and green). Green - ab125025 observed at 175 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

    ab125025 was shown to react with ROCK2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265679 (knockout cell lysate ab257643) was used. Wild-type HeLa and ROCK2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab125025 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ROCK2 antibody [EPR7141(B)] (AB125025) at 1/10000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ROCK2 knockout HeLa cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: A549 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 161 kDa

    Observed band size: 175 kDa

  • Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025), expandable thumbnail

    Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)

    Blocking and Diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-ROCK2 antibody [EPR7141(B)] (AB125025) at 1/50000 dilution

    Lane 1: HeLa whole cell lysate at 10 µg

    Lane 2: HEK293 whole cell lysate at 10 µg

    Lane 3: Mouse brain tissue lysate at 10 µg

    Lane 4: Rat brain tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 161 kDa

    Observed band size: 161 kDa

  • Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025), expandable thumbnail

    Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)

    All lanes: Western blot - Anti-ROCK2 antibody [EPR7141(B)] (AB125025) at 1/10000 dilution

    Lane 1: Hela cell lysate at 10 µg

    Lane 2: L6 cell lysate at 10 µg

    Lane 3: A673 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 161 kDa

  • Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025), expandable thumbnail

    Western blot - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)

    Blocking and Diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-ROCK2 antibody [EPR7141(B)] (AB125025) at 1/10000 dilution

    All lanes: RAW264.7 whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 161 kDa, 305 kDa

    Observed band size: 161 kDa

  • OI-RD Scanning - Anti-ROCK2 antibody [EPR7141(B)] (ab125025), expandable thumbnail

    OI-RD Scanning - Anti-ROCK2 antibody [EPR7141(B)] (ab125025)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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