Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--western-blot-img138278.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961)")
![Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--immunocytochemistry-immunofluorescence-img123069.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961)")
![Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--western-blot-img136709.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961)")
![OI-RD Scanning - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--oi-rd-scanning-img85116.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (AB238961)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Tested | Not recommended | Expected |
| Rat | Tested | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
2 products for Alternative Version
Target data
Function
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
Alternative names
KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II
Recommended products
Rabbit Recombinant Monoclonal ROCK2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7141(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238961 is the carrier-free version of Anti-ROCK2 antibody [EPR7141(B)] ab125025.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--western-blot-img138278.jpg "Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)")
Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: ROCK2 knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: A10 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-ROCK2 antibody [EPR7141(B)] ab125025 observed at 165 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
Anti-ROCK2 antibody [EPR7141(B)] ab125025 was shown to specifically react with ROCK2 when ROCK2 knockout samples were used. Wild-type and ROCK2 knockout samples were subjected to SDS-PAGE. Anti-ROCK2 antibody [EPR7141(B)] ab125025 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-ROCK2 antibody [EPR7141(B)] ab125025).
All lanes: Western blot - Anti-ROCK2 antibody [EPR7141(B)] (Anti-ROCK2 antibody [EPR7141(B)] ab125025)
Predicted band size: 161 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--immunocytochemistry-immunofluorescence-img123069.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)")
Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling ROCK2 with purified Anti-ROCK2 antibody [EPR7141(B)] ab125025 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 anti-Tubulin (mouse mAb) primary and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 goat anti-mouse) secondary both at 1/1000 dilution. Nuclei were counterstained with DAPI (blue).
For negative control 1, rabbit primary antibody and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (anti-mouse) secondary antibody were used. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse primary antibody) was used followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (anti-rabbit secondary antibody).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ROCK2 antibody [EPR7141(B)] ab125025).
![Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--western-blot-img136709.jpg "Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)")
Western blot - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ROCK2 antibody [EPR7141(B)] ab125025).
Lanes 1- 4: Merged signal (red and green). Green - Anti-ROCK2 antibody [EPR7141(B)] ab125025 observed at 175 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-ROCK2 antibody [EPR7141(B)] ab125025 was shown to react with ROCK2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human ROCK2 knockout HeLa cell line ab265679 (knockout cell lysate Human ROCK2 knockout HeLa cell lysate ab257643) was used. Wild-type HeLa and ROCK2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-ROCK2 antibody [EPR7141(B)] ab125025 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ROCK2 antibody [EPR7141(B)] (Anti-ROCK2 antibody [EPR7141(B)] ab125025) at 1/10000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ROCK2 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ROCK2 knockout HeLa cell line (Human ROCK2 knockout HeLa cell line ab265679)
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 161 kDa
Observed band size: 175 kDa
![OI-RD Scanning - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961), expandable thumbnail](https://content.abcam.com/products/images/rock2-antibody-epr7141b-bsa-and-azide-free-ab238961--oi-rd-scanning-img85116.jpg "OI-RD Scanning - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)")
OI-RD Scanning - Anti-ROCK2 antibody [EPR7141(B)] - BSA and Azide free (ab238961)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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