Rabbit Polyclonal ROCK2 phospho S1366 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ROCK2 phospho S1366.
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- Frontiers in cardiovascular medicine 10:11918912023Apelin prevents diabetes-induced poor collateral vessel formation and blood flow reperfusion in ischemic limb.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStéphanie Robillard,Kien Trân,Marie-Sophie Lachance,Tristan Brazeau,Elizabeth Boisvert,Farah Lizotte,Mannix Auger-Messier,Pierre-Luc Boudreault,Éric Marsault,Pedro GeraldesPubMed 37636297
- Nature communications 14:38872023SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Tan,Meiping Lu,Yuqing Cai,Weibo Qi,Fugen Wu,Hangyang Bao,Meiyu Qv,Qiangqiang He,Yana Xu,Xiangzhi Wang,Tingyu Shen,Jiahao Luo,Yangxun He,Junsong Wu,Lanfang Tang,Muhammad Qasim Barkat,Chengyun Xu,Ximei WuPubMed 37393345
- Cell reports 40:1110802022Optical regulation of endogenous RhoA reveals selection of cellular responses by signal amplitude.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeongmin Ju,Hae Nim Lee,Lin Ning,Hyunjoo Ryu,Xin X Zhou,Hyeyeon Chun,Yong Woo Lee,Austin I Lee-Richerson,Cherlhyun Jeong,Michael Z Lin,Jihye SeongPubMed 35830815
- Cell 185:1356-1372.e262022Tumor-resident intracellular microbiota promotes metastatic colonization in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAikun Fu,Bingqing Yao,Tingting Dong,Yongyi Chen,Jia Yao,Yu Liu,Hang Li,Huiru Bai,Xiaoqin Liu,Yue Zhang,Chunhui Wang,Yajing Guo,Nan Li,Shang CaiPubMed 35395179
- Cell cycle (Georgetown, Tex.) 21:340-3512022Nc886 promotes renal cancer cell drug-resistance by enhancing EMT through Rock2 phosphorylation-mediated β-catenin nuclear translocation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiyin Gao,Shouhua Zhang,Li Guorong,Queling Liu,Anyi Zhu,Fu Gui,Yan Zou,Yiguo Wu,Yang Luo,Zhengdong HongPubMed 34974812
- Cellular and molecular neurobiology :2020A Progressive Loss of phosphoSer138-Profilin Aligns with Symptomatic Course in the R6/2 Mouse Model of Huntington's Disease: Possible Sex-Dependent Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkanksha Baharani,Zelan Wei,William J Roesler,Darrell D MousseauPubMed 33108594
- RSC advances 8:34973-349832018Retracted Article: MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiming Yang,Zhi Chen,Daguang Fan,Rui Zhang,Yuhong Zhang,Shusheng WuPubMed 35547072
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ROCK2 phospho S1366. The exact immunogen used to generate this antibody is proprietary information. Database link O75116
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
Alternative names
KIAA0619, ROCK2, Rho-associated protein kinase 2, Rho kinase 2, p164 ROCK-2, ROCK-II
Recommended products
Rabbit Polyclonal ROCK2 phospho S1366 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ROCK2 phospho S1366.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ROCK2 phospho S1366. The exact immunogen used to generate this antibody is proprietary information. Database link O75116
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/500 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lanes 2 - 3: ROCK2 shRNA transfected HEK-293T whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 161 kDa
Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008)
5% SDS-PAGE gel.
All lanes: Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/2000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with CIP (calf-intestinal alkaline phosphatase) at 30 µg
Predicted band size: 161 kDa
Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008)
HEK-293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCK2 expression constructs. Overexpressed Myc-ROCK2 proteins were immunoprecipitated with anti-myc antibody and probed with ab228008 with or without peptide (0.2 mg/ml) competition as indicated.
All lanes: Western blot - Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/1000 dilution
Lanes 1, 3 and 5: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with WT ROCK2, whole cell lysate
Lanes 2, 4 and 6: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with phospho-mutant (S1366A) ROCK2, whole cell lysate
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 161 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (ab228008)
Paraffin-embedded HBL435 xenograft tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (ab228008)
Paraffin-embedded human breast tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (ab228008)
Paraffin-embedded mouse brain tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 (phospho S1366) antibody (ab228008)
Paraffin-embedded rat kidney tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 (phospho S1366) antibody (ab228008)
Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROCK2 (phospho S1366) (green) using ab228008 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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