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AB317080

Anti-ROCK2 (phospho S1366) antibody [HL1071]

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Rabbit Monoclonal antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ROCK2 phospho S1366 conjugated to Keyhole Limpet Haemocyanin.
3 Images
Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (AB317080)
  • WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (AB317080)

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ab317080 diluted at 1 : 1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (ab317080) at 1/1000 dilution

All lanes:

HeLa cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (AB317080)
  • WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (AB317080)

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ab317080 diluted at 1 : 1000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (ab317080) at 1/1000 dilution

Lane 1:

Untreated (–) A431 whole cell extracts at 30 µg

Lane 2:

Treated (+) A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (AB317080)
  • WB

Supplier Data

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (AB317080)

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ab317080 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ROCK2 (phospho S1366) antibody [HL1071] (ab317080) at 1/1000 dilution

Lane 1:

Untreated (-) HeLa whole cell extracts at 30 µg

Lane 2:

Treated (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1071

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ROCK2 phospho S1366 conjugated to Keyhole Limpet Haemocyanin.

O75116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
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Product promise

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