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Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) is a rabbit monoclonal antibody that is used to detect ROCK1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Cow, Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 130 publications

-Over 130 publications


Images

Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171), expandable thumbnail
  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171), expandable thumbnail
  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-PIHC-Fr
Human
Tested
Tested
Tested
Tested
Tested
Predicted
Mouse
Expected
Tested
Expected
Expected
Expected
Predicted
Rat
Expected
Tested
Expected
Expected
Expected
Predicted
Cow
Predicted
Expected
Predicted
Predicted
Predicted
Expected
Recombinant fragment - Human
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/40 - 1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/2000 - 1/10000
Notes

For unpurified use at 1/500.

Species
Mouse
Dilution info
1/2000 - 1/10000
Notes

For unpurified use at 1/500.

Species
Rat
Dilution info
1/2000 - 1/10000
Notes

For unpurified use at 1/500.

Species
Human
Dilution info
1/2000 - 1/10000
Notes

For unpurified use at 1/500.

Expected
Expected

Species
Cow
Dilution info
1/2000 - 1/10000
Notes

For unpurified use at 1/500.

Tested
Tested

Species
Human
Dilution info
1/50 - 1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed:10436159, PubMed:10652353, PubMed:11018042, PubMed:11283607, PubMed:17158456, PubMed:18573880, PubMed:19131646, PubMed:8617235, PubMed:9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed:10436159, PubMed:10652353, PubMed:11018042, PubMed:11283607, PubMed:17158456, PubMed:18573880, PubMed:19131646, PubMed:23093407, PubMed:23355470, PubMed:8617235, PubMed:9722579). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed:18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed:19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed:19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed:21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed:19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity).

Additional Targets

ROCK2

Alternative names

Recommended products

Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) is a rabbit monoclonal antibody that is used to detect ROCK1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Cow, Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 130 publications

-Over 130 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP786Y
Purification technique
Affinity purification Protein A
Specificity

This antibody recognizes both the cleaved C-terminus of ROCK 1 (30 kDa) and full length protein (158 kDa). The immunogen used for this product shares 83% homology with ROCK2 and has been shown to bind recombinant human ROCK2, please see western blot images below.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), in Cow, Human, Mouse, Rat samples.

What is the molecular weight of ROCK1?


Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) specifically detects a band for ROCK1 (UniProt: Q13464) at a molecular weight of 158kDa.

Recommended positive controls


WB: Untreated, Calyculin A treated and Camptothecin treated HeLa whole cell lysate (HeLa whole cell lysate ab150035). Jurkat, Ramos, PC-12 and RAW264.7 cell lysates.IHC-P: Human adenocarcinoma of the colon and thyroid gland carcinoma tissues.ICC/IF: HeLa cells.Flow Cyt (intra): HeLa cells.IP: HeLa whole cell lysate (HeLa whole cell lysate ab150035).

Trusted by the scientific community


Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) was first used in a scientific publication in 2006 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists


Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EP786Y] also available for your convenience:
ab45171, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] ab205432, Carrier free - Anti-ROCK2 + ROCK1 antibody [EP786Y] - BSA and Azide free ab219587, PE - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] ab224972, APC - APC Anti-ROCK1 antibody [EP786Y] ab310864, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-ROCK1 antibody [EP786Y] ab311078, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-ROCK1 antibody [EP786Y] ab311669, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-ROCK2 + ROCK1 antibody [EP786Y] ab312944, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-ROCK1 antibody [EP786Y] ab313154, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-ROCK2 + ROCK1 antibody [EP786Y] ab321633



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    ab45171 (purified) at 1/40 immunoprecipitating ROCK2 + ROCK1 in HeLa cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Observed band size: 158 kDa

  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution

    Lane 1: HeLa cell lysate - treated with Calyculin A at 20 µg

    Lane 2: HeLa cell lysate - treated with Camptothecin at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Lane 5: Ramos cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 158 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ROCK2 + ROCK1 with purified ab45171 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Loading control: Anti-6X His tag® antibody [EPR20547] (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204)

    Blocking buffer and concentration: 5% NFDM/TBST

    Exposure Times:

    Lane 1: 3 seconds

    Lane 2: 7 seconds

    Lanes 1 - 2: Western blot - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] ab205432) at 1/1000 dilution

    Lanes 1 - 2: Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (PE Anti-ROCK2 + ROCK1 antibody [EP786Y] ab224972) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/1000 dilution

    Lane 1: Recombinant Human ROCK1 protein (aa 1114 to 1354) (30 kDa)

    Lane 2: Recombinant Human ROCK2 protein (aa 1132 to 1388) (30 kDa)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of the colon tissue labelling ROCK2 + ROCK1 with purified ab45171 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution

    Lane 1: PC-12 cell lysate at 20 µg

    Lane 2: RAW264.7 cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 158 kDa

  • Flow Cytometry (Intracellular) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Overlay histogram showing HeLa cells stained with unpurified ab45171 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45171, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling ROCK2 + ROCK1 with unpurified ab45171.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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