Anti-ROCK2 + ROCK1 antibody [EP786Y]
- RabMAb
- Recombinant
- What is this?
4
(9 Reviews)
|
(133 Publications)
Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) is a rabbit monoclonal antibody detecting ROCK1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Cow, Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 130 publications
- Trusted since 2006
View Alternative Names
Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, p160 ROCK-1, ROCK-I, p160ROCK, ROCK1
- WB
Lab
Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution
Lane 1:
HeLa cell lysate - treated with Calyculin A at 20 µg
Lane 2:
HeLa cell lysate - treated with Camptothecin at 20 µg
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Lane 5:
Ramos cell lysate at 20 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Observed band size: 158 kDa
false
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling ROCK2 + ROCK1 with unpurified ab45171.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
Overlay histogram showing HeLa cells stained with unpurified ab45171 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45171, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ROCK2 + ROCK1 with purified ab45171 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control : primary antibody (1/50) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of the colon tissue labelling ROCK2 + ROCK1 with purified ab45171 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- IP
Unknown
Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
ab45171 (purified) at 1/40 immunoprecipitating ROCK2 + ROCK1 in HeLa cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
Observed band size: 158 kDa
false
- WB
Supplier Data
Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
Loading control : Anti-6X His tag® antibody [EPR20547] (ab213204)
Blocking buffer and concentration : 5% NFDM/TBST
Exposure Times :
Lane 1 : 3 seconds
Lane 2 : 7 seconds
Lanes 1 - 2:
Western blot - Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y] (<a href='/en-us/products/primary-antibodies/alexa-fluor-488-rock2-rock1-antibody-ep786y-ab205432'>ab205432</a>) at 1/1000 dilution
Lanes 1 - 2:
Western blot - PE Anti-ROCK2 + ROCK1 antibody [EP786Y] (<a href='/en-us/products/primary-antibodies/pe-rock2-rock1-antibody-ep786y-ab224972'>ab224972</a>) at 1/1000 dilution
Lanes 1 - 2:
Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/1000 dilution
Lane 1:
Recombinant Human ROCK1 protein (aa 1114 to 1354) (30 kDa)
Lane 2:
Recombinant Human ROCK2 protein (aa 1132 to 1388) (30 kDa)
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 30 kDa
false
- WB
Lab
Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (AB45171)
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution
Lane 1:
PC-12 cell lysate at 20 µg
Lane 2:
RAW264.7 cell lysate at 20 µg
Secondary
All lanes:
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Observed band size: 158 kDa
false
Related conjugates and formulations (8)
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-ROCK2 + ROCK1 antibody [EP786Y]
-
578 PE
PE Anti-ROCK2 + ROCK1 antibody [EP786Y]
-
Anti-ROCK2 + ROCK1 antibody [EP786Y] - BSA and Azide free
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-ROCK1 antibody [EP786Y]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-ROCK1 antibody [EP786Y]
-
660 APC
APC Anti-ROCK1 antibody [EP786Y]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-ROCK2 + ROCK1 antibody [EP786Y]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-ROCK2 + ROCK1 antibody [EP786Y]
Reactivity data
Product details
What is this antibody validated in?
Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.
What is the molecular weight of ROCK1?
Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) specifically detects a band for ROCK1 (UniProt: Q13464) at a molecular weight of 158kDa.
Trusted by the scientific community
Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) was first used in a scientific publication in 2006 and has been cited over 130 times in peer-reviewed journals.
Reviewed by scientists
Anti-ROCK2 + ROCK1 [EP786Y] (ab45171) has over 5 independent reviews from customers.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EP786Y] also available for your convenience: ab45171, Alexa Fluor® 488 - ab205432, Carrier free - ab219587, PE - ab224972, APC - ab310864, Alexa Fluor® 647 - ab311078, Alexa Fluor® 594 - ab311669, Alexa Fluor® 568 - ab312944, Alexa Fluor® 555 - ab313154, Alexa Fluor® 750 - ab321633
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Additional targets
Publications (133)
Recent publications for all applications. Explore the full list and refine your search
Journal of vascular research 60:183-192 PubMed37660689
2023
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23035 PubMed37310396
2023
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Phytotherapy research : PTR 37:4117-4132 PubMed37246830
2023
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Respiratory research 24:56 PubMed36803977
2023
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Nature communications 14:700 PubMed36755029
2023
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Cancers 14: PubMed35626071
2022
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Journal of cellular and molecular medicine 26:1656-1671 PubMed35092120
2022
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Aging 14:286-296 PubMed34983026
2022
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Evidence-based complementary and alternative medicine : eCAM 2021:5536679 PubMed34925530
2021
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Frontiers in cell and developmental biology 9:796274 PubMed34957122
2021
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Product promise
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