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AB236407

Anti-ROD1 antibody [OTI5G7]

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(2 Publications)

Mouse Monoclonal ROD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTBP3 aa 300 to C-terminus.

View Alternative Names

ROD1, PTBP3, Polypyrimidine tract-binding protein 3, Regulator of differentiation 1, Rod1

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human colon tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human spleen tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human liver tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human breast tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human ovary adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human colon adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human thyroid tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human breast adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human prostate carcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (AB236407)

Paraffin-embedded human kidney tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • WB

Supplier Data

Western blot - Anti-ROD1 antibody [OTI5G7] (AB236407)

All lanes:

Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY ROD1 cDNA transfected HEK-293T cell lysate at 5 µg

Predicted band size: 60 kDa

false

Western blot - Anti-ROD1 antibody [OTI5G7] (AB236407)
  • WB

Supplier Data

Western blot - Anti-ROD1 antibody [OTI5G7] (AB236407)

All lanes:

Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Lane 2:

A549 (human lung carcinoma cell line) cell lysate at 35 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

Predicted band size: 60 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI5G7

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PTBP3 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95758

Specificity

The immunogen used for this product shares 75% homology with PTBP1 and 68% homology with PTBP2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH 8.5) at 120°C for 3 minutes.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U).
See full target information PTBP3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular carcinogenesis 62:907-919 PubMed37157950

2023

PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Yunli Zhang,Shiyu Pang,Jingxian Min,Tao Wang,Dali Wu,Chun Lin,Zebin Xiao,Qi Xiang,Qing Li,Lili Ma

Journal of digestive diseases 23:376-387 PubMed35844201

2022

Circular RNA circ_0006089 promotes the progression of gastric cancer by regulating the miR-143-3p/PTBP3 axis and PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Rong Lin,Wei Rong Chen,Pei Hong Zheng,Wei Shan Chen,Gao Yang Cai
View all publications
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Product promise

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