Anti-ROD1 antibody [OTI5G7] (ab236407)

Mouse Monoclonal ROD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PTBP3 aa 300 to C-terminus.

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Images

Western blot - Anti-ROD1 antibody [OTI5G7] (AB236407), expandable thumbnail

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human PTBP3 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95758

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P
Human	Tested	Tested
Mouse	Predicted	Predicted
Rat	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500 - 1/2000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH 8.5) at 120°C for 3 minutes.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Target data

See full target information PTBP3

Function

RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U).

Alternative names

ROD1, PTBP3, Polypyrimidine tract-binding protein 3, Regulator of differentiation 1, Rod1

Recommended products

Mouse Monoclonal ROD1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PTBP3 aa 300 to C-terminus.

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human PTBP3 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95758
Clone number: OTI5G7
Purification technique: Affinity purification
Specificity: The immunogen used for this product shares 75% homology with PTBP1 and 68% homology with PTBP2. Cross-reactivity with this protein has not been confirmed experimentally.
Concentration
Purification notes: Purified from cell culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Product promise

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Full details and terms and conditions can be found here:
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13 product images

Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407)
All lanes: Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/2000 dilution
Lane 1: pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg
Lane 2: pCMV6-ENTRY ROD1 cDNA transfected HEK-293T cell lysate at 5 µg
Predicted band size: 60 kDa
Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407)
All lanes: Western blot - Anti-ROD1 antibody [OTI5G7] (ab236407) at 1/500 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg
Lane 2: A549 (human lung carcinoma cell line) cell lysate at 35 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human breast tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human breast adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human colon tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human colon adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human kidney tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human liver tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human ovary adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human thyroid tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human endometrium adenocarcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human prostate carcinoma tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROD1 antibody [OTI5G7] (ab236407)
Paraffin-embedded human spleen tissue stained for ROD1 using ab236407 at 1/500 dilution in immunohistochemical analysis.