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Rabbit Recombinant Monoclonal ROGDI antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-ROGDI antibody [EPR15190] - BSA and Azide free (AB250791), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ROGDI antibody [EPR15190] - BSA and Azide free (AB250791), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ROGDI antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15190
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250791 is the carrier-free version of Anti-ROGDI antibody [EPR15190] ab184954.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-ROGDI antibody [EPR15190] - BSA and Azide free (ab250791), expandable thumbnail

    Western blot - Anti-ROGDI antibody [EPR15190] - BSA and Azide free (ab250791)

    This data was developed using Anti-ROGDI antibody [EPR15190] ab184954, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ROGDI antibody [EPR15190] (Anti-ROGDI antibody [EPR15190] ab184954) at 1/20000 dilution

    Lane 1: Human fetal brain lysate at 20 µg

    Lane 2: Human bone marrow lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 32 kDa

    Observed band size: 32 kDa

  • Flow Cytometry (Intracellular) - Anti-ROGDI antibody [EPR15190] - BSA and Azide free (ab250791), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ROGDI antibody [EPR15190] - BSA and Azide free (ab250791)

    This data was developed using Anti-ROGDI antibody [EPR15190] ab184954, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling ROGDI with Anti-ROGDI antibody [EPR15190] ab184954 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com