Anti-ROMO1 antibody [OTI2C12] (ab236409)

Mouse Monoclonal ROMO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Human, Mouse, Dog, African green monkey, Human, Transfected cell line - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ROMO1.

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Images

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409), expandable thumbnail

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Full Length Protein corresponding to Human ROMO1. Database link P60602

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Expected
African green monkey	Tested	Expected	Expected
Cow	Predicted	Predicted	Predicted
Dog	Tested	Expected	Expected
Pig	Predicted	Predicted	Predicted
Transfected cell line - Human	Not recommended	Not recommended	Tested
Transfected cell lysate - Human	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info 1/2000	Notes -
Species Mouse	Dilution info 1/2000	Notes -
Species Dog	Dilution info 1/2000	Notes -
Species African green monkey	Dilution info 1/2000	Notes -
Species Human	Dilution info 1/2000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Cow, Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/150	Notes Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Dog	Dilution info -	Notes -
Species African green monkey	Dilution info 1/150	Notes Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes

Predicted
Predicted

Species	Dilution info	Notes
Species Cow, Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human, Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info 1/100	Notes -
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Dog	Dilution info -	Notes -
Species African green monkey	Dilution info 1/100	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Cow, Pig	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Target data

See full target information ROMO1

Function

Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation. Has antibacterial activity against a variety of bacteria including S.aureus, P.aeruginosa and M.tuberculosis. Acts by inducing bacterial membrane breakage.

Alternative names

C20orf52, ROMO1, Reactive oxygen species modulator 1, ROS modulator 1, Epididymis tissue protein Li 175, Glyrichin, Mitochondrial targeting GxxxG motif protein, Protein MGR2 homolog, MTGM

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Full Length Protein corresponding to Human ROMO1. Database link P60602
Clone number: OTI2C12
Purification technique: Affinity purification
Concentration
Purification notes: Purified from cell culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Clone OTI2C12 (formerly 2C12)

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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12 product images

Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
All lanes: Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate at 5 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ROMO1, whole cell lysate at 5 µg
Predicted band size: 8 kDa
Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
All lanes: Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 3: SV-T2 (Mouse cell line) whole cell lysate at 10 µg
Lane 4: A549 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 10 µg
Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg
Lane 7: MDCK (canine kidney cell line) whole cell lysate at 10 µg
Predicted band size: 8 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human breast adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human colon adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human kidney tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human ovary adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human pancreas tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human thyroid tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human endometrial adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human prostate tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ROMO1 stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.
Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.