AB236409

Anti-ROMO1 antibody [OTI2C12]

Name: Anti-ROMO1 antibody [OTI2C12] (ab236409) | Abcam
Brand: Abcam Limited
SKU: AB236409

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(4 Publications)

Mouse Monoclonal ROMO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Human, Mouse, Dog, African green monkey, Human, Transfected cell line - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ROMO1.

View Alternative Names

C20orf52, ROMO1, Reactive oxygen species modulator 1, ROS modulator 1, Epididymis tissue protein Li 175, Glyrichin, Mitochondrial targeting GxxxG motif protein, Protein MGR2 homolog, MTGM

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human thyroid tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human pancreas tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human endometrial adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human colon adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human kidney tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human breast adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human prostate tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Paraffin-embedded human ovary adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

All lanes:

Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

SV-T2 (Mouse cell line) whole cell lysate at 10 µg

Lane 4:

A549 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 10 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 7:

MDCK (canine kidney cell line) whole cell lysate at 10 µg

Predicted band size: 8 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ROMO1 stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.

Supplier Data

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

All lanes:

Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate at 5 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ROMO1, whole cell lysate at 5 µg

Predicted band size: 8 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2C12

Isotype

IgG1

Carrier free

Reacts with

Mouse, Dog, Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ROMO1.

P60602

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Clone OTI2C12 (formerly 2C12)

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coordination of mitochondrial morphology and cell proliferation.. Has antibacterial activity against a variety of bacteria including S.aureus, P.aeruginosa and M.tuberculosis. Acts by inducing bacterial membrane breakage.

See full target information ROMO1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Animal bioscience 38:303-315 PubMed39210798

2024

shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Cheng,Shu Zhen Jiang,Li Bo Huang,Wei Ren Yang

Toxins 14: PubMed36422967

2022

Zearalenone Exposure Affects the Keap1-Nrf2 Signaling Pathway and Glucose Nutrient Absorption Related Genes of Porcine Jejunal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Cheng,Shuzhen Jiang,Libo Huang,Yuxi Wang,Weiren Yang

Journal of digestive diseases 23:376-387 PubMed35844201

2022

Circular RNA circ_0006089 promotes the progression of gastric cancer by regulating the miR-143-3p/PTBP3 axis and PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Rong Lin,Wei Rong Chen,Pei Hong Zheng,Wei Shan Chen,Gao Yang Cai

Cellular and molecular neurobiology 42:2379-2392 PubMed34089427

2021

GPR18 Agonist Resolvin D2 Reduces Early Brain Injury in a Rat Model of Subarachnoid Hemorrhage by Multiple Protective Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Tongyu Zhang,Gang Zuo,Hongqi Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ROMO1 antibody [OTI2C12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Western blot - Anti-ROMO1 antibody [OTI2C12] (AB236409)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise