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AB52927

Anti-RON antibody [EP1132Y]

3

(1 Review)

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(12 Publications)

Rabbit Recombinant Monoclonal RON phospho Y1353 antibody. Suitable for IHC-P, IP, ELISA, WB, Flow Cyt (Intra) and reacts with Human, Recombinant fragment, Recombinant full length protein - Human samples. Cited in 12 publications.

View Alternative Names

CD136, PTK8, RON, MST1R, Macrophage-stimulating protein receptor, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (AB52927)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (AB52927)

Immunohistochemical staining of paraffin-embedded human stomach tissue using ab52927 at a dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-RON antibody [EP1132Y] (AB52927)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-RON antibody [EP1132Y] (AB52927)

Overlay histogram showing THP1 cells stained with ab52927 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52927 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1μg/1x106 cells) used under the same conditions. Acquisition of >5000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.

ELISA - Anti-RON antibody [EP1132Y] (AB52927)
  • ELISA

Lab

ELISA - Anti-RON antibody [EP1132Y] (AB52927)

ELISA analysis of Human RON recombinant protein at 1000 ng/mL with ab52927. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Western blot - Anti-RON antibody [EP1132Y] (AB52927)
  • WB

Unknown

Western blot - Anti-RON antibody [EP1132Y] (AB52927)

All lanes:

Western blot - Anti-RON antibody [EP1132Y] (ab52927) at 1/10000 dilution

All lanes:

SKBR3 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit

Predicted band size: 152 kDa

false

  • Carrier free

    Anti-RON antibody [EP1132Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1132Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ELISA, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p>1/10000. Detects a band of approximately 35/150/180 kDa (predicted molecular weight: 185 kDa). See Notes section of Datasheet.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20 - 1/40", "FlowCytIntra-species-notes": "<p>Methanol fixed cells. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Recombinant fragment": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
See full target information MST1R phospho Y1353
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Contemporary oncology (Poznan, Poland) 26:109-122 PubMed35903204

2022

RON, ROR1 and SUSD2 expression in tissues of endometrial carcinoma patients. Clinicopathological and prognostic implications.

Applications

Unspecified application

Species

Unspecified reactive species

Abeer M Abdelbary,Randa Mohamed Kaf,Mohamed Elbakry Lashin,Ahmed Z Alattar,Dalia Hamouda Elsayed,Ahmed F Amin,Loay M Gertallah,Ahmed Mohamed Yehia

BioMed research international 2021:2282916 PubMed34712728

2021

RON Mediates Tumor-Promoting Effects in Endometrial Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Yu,Jianzhang Wang,Tiantian Li,Xinxin Xu,Xinyue Guo,Shaojie Ding,Libo Zhu,Gen Zou,Yichen Chen,Xinmei Zhang

Experimental and therapeutic medicine 22:798 PubMed34093754

2021

The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR.

Applications

Unspecified application

Species

Unspecified reactive species

Donghui Zhou,Xiaohua Zhu,Xuan Wu,Jingjing Zheng,Laizhen Tou,Yong Zhou

Journal of cellular and molecular medicine 25:1601-1612 PubMed33410267

2021

Recepteur d'origine nantais contributes to the development of endometriosis via promoting epithelial-mesenchymal transition of a endometrial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Yu,Jianzhang Wang,Tiantian Li,Xinyue Guo,Shaojie Ding,Xuan Che,Libo Zhu,Yangying Peng,Xinxin Xu,Gen Zou,Xinmei Zhang

Scientific reports 9:18101 PubMed31792339

2019

OPN promotes the aggressiveness of non-small-cell lung cancer cells through the activation of the RON tyrosine kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Hao,Yuxin Cui,Siyuan Chang,Jing Huang,Emily Birkin,Mu Hu,Xiuyi Zhi,Wenbin Li,Lijian Zhang,Shan Cheng,Wen G Jiang

Oncology letters 14:4225-4230 PubMed28943931

2017

A missing link between RON expression and oncological outcomes in resected left-sided pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dai Hoon Han,Chang Moo Kang,Sung Whan Lee,Ho Kyoung Hwang,Woo Jung Lee

Investigational new drugs 35:442-450 PubMed28161886

2017

Phase 1 study of narnatumab, an anti-RON receptor monoclonal antibody, in patients with advanced solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia M LoRusso,Mrinal Gounder,Shadia I Jalal,Valérie André,Siva Rama Prasad Kambhampati,Nick Loizos,Jennifer Hall,Timothy R Holzer,Aejaz Nasir,Jan Cosaert,John Kauh,E Gabriela Chiorean

Anticancer research 36:6585-6592 PubMed27919987

2016

The Complete Loss of Tyrosine Kinase Receptors MET and RON Is a Poor Prognostic Factor in Patients with Extrahepatic Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Hayashi,Junpei Yamaguchi,Toshio Kokuryo,Tomoki Ebata,Yukihiro Yokoyama,Tsuyoshi Igami,Gen Sugawara,Masato Nagino

PloS one 11:e0161626 PubMed27559727

2016

Plasma Level of Placenta-Derived Macrophage-Stimulating Protein -Chain in Preeclampsia before 20 Weeks of Pregnancy.

Applications

IHC-P

Species

Unspecified reactive species

Yonggang Zhang,Hongling Yang,Yan Long,Qingling Ma,Ruihua Chen

Cancer letters 340:43-50 PubMed23811285

2013

RON confers lapatinib resistance in HER2-positive breast cancer cells.

Applications

WB

Species

Human

Quanren Wang,Haitian Quan,Jie Zhao,Chengying Xie,Lei Wang,Liguang Lou
View all publications
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