Rabbit Recombinant Monoclonal RON phospho Y1353 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (AB251281), expandable thumbnail](https://content.abcam.com/products/images/ron-antibody-epr16789-bsa-and-azide-free-ab251281--western-blot-img83491.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (AB251281)")
![Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (AB251281), expandable thumbnail](https://content.abcam.com/products/images/ron-antibody-epr16789-bsa-and-azide-free-ab251281--western-blot-img19005.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (AB251281)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
Alternative names
CD136, PTK8, RON, MST1R, Macrophage-stimulating protein receptor, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron
Recommended products
Rabbit Recombinant Monoclonal RON phospho Y1353 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16789
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251281 is the carrier-free version of Anti-RON antibody [EPR16789] ab199532.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281), expandable thumbnail](https://content.abcam.com/products/images/ron-antibody-epr16789-bsa-and-azide-free-ab251281--western-blot-img83491.jpg "Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)")
Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)
This data was developed using Anti-RON antibody [EPR16789] ab199532, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 17616662).
All lanes: Western blot - Anti-RON antibody [EPR16789] (Anti-RON antibody [EPR16789] ab199532) at 1/4000 dilution
Lane 1: HT-29 (Human colorectal adenocarcinoma cells) whole cell lysate at 10 µg
Lane 2: BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 152 kDa
Observed band size: 185 kDa
Exposure time: 30s
![Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281), expandable thumbnail](https://content.abcam.com/products/images/ron-antibody-epr16789-bsa-and-azide-free-ab251281--western-blot-img19005.jpg "Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)")
Western blot - Anti-RON antibody [EPR16789] - BSA and Azide free (ab251281)
This data was developed using Anti-RON antibody [EPR16789] ab199532, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Both MCF7 and MDA MB 435S are RON negative cell lines.
All lanes: Western blot - Anti-RON antibody [EPR16789] (Anti-RON antibody [EPR16789] ab199532) at 1/1000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 2: MDA-MB-435S (Human ductal carcinoma) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 152 kDa
Exposure time: 3min
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