JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB124671

Anti-RON (phospho Y1353) antibody [EPR5598]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal RON phospho Y1353 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD136, PTK8, RON, MST1R, Macrophage-stimulating protein receptor, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

ab124671, at 1/500 dilution, staining RON in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.

Western blot - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)
  • WB

Unknown

Western blot - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

All lanes:

Western blot - Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671) at 1/50000 dilution

Lane 1:

A431 cell lysates, untreated

Lane 2:

A431 cell lysates, treated with pervanadate

Predicted band size: 152 kDa

false

OI-RD Scanning - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-RON (phospho Y1353) antibody [EPR5598] (AB124671)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5598

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
See full target information MST1R phospho Y1353
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:351 PubMed35428350

2022

EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli.

Applications

Unspecified application

Species

Unspecified reactive species

Shouyang Song,Zhen Yu,Yajing You,Chenxi Liu,Xiaoyu Xie,Huanran Lv,Feng Xiao,Qiang Zhu,Chengyong Qin

Experimental and therapeutic medicine 22:798 PubMed34093754

2021

The effect of splicing MST1R in gastric cancer was enhanced by lncRNA FENDRR.

Applications

Unspecified application

Species

Unspecified reactive species

Donghui Zhou,Xiaohua Zhu,Xuan Wu,Jingjing Zheng,Laizhen Tou,Yong Zhou

PloS one 13:e0191983 PubMed29408900

2018

Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia de la Fuente,Chelsea Pinkham,Deemah Dabbagh,Brett Beitzel,Aura Garrison,Gustavo Palacios,Kimberley Alex Hodge,Emanuel F Petricoin,Connie Schmaljohn,Catherine E Campbell,Aarthi Narayanan,Kylene Kehn-Hall
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB203752

Anti-RON (phospho Y1238) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON (phospho Y1238) antibody (AB203752)

  • 0
  • 0
Primary Antibodies

AB199532

Anti-RON antibody [EPR16789]

RabMAb|Recombinant

Western blot - Anti-RON antibody [EPR16789] (AB199532)

  • 0
  • 0
Primary Antibodies

AB227979

Anti-RON antibody [EPR20954]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] (AB227979)

  • 0
  • 0
Primary Antibodies

AB228853

Anti-RON antibody [EPR20954] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20954] - BSA and Azide free (AB228853)

  • 0
  • 0
Primary Antibodies

AB228855

Anti-RON antibody [EPR20953] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] - BSA and Azide free (AB228855)

  • 0
  • 0
Primary Antibodies

AB52927

Anti-RON antibody [EP1132Y]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (AB52927)

  • 3
  • 1
Primary Antibodies

AB227980

Anti-RON antibody [EPR20953]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EPR20953] (AB227980)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB51103

Anti-Ret (phospho Y1062) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ret (phospho Y1062) antibody (AB51103)

  • 0
  • 0
Primary Antibodies

AB255607

Anti-ErbB3 / HER3 antibody [EPR22669-25]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB3 / HER3 antibody [EPR22669-25] (AB255607)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB237781

Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free

RabMAb|Recombinant

Dot Blot - Anti-PDGFR beta (phospho Y751) antibody [EPR21744] - BSA and Azide free (AB237781)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com